Functional Expression of Cone Cyclic Nucleotide-Gated Channel in Cone Photoreceptor-Derived 661W Cells
Cone phototransduction mediated by cone cyclic nucleotide-gated (CNG) channel activation is essential for central and color vision. Rod or cone CNG channels are composed of two structurally related subunit types: the A and the B subunits. The stoichiometry of the rod CNG channel complex has been established by biochemical, biophysical and molecular biological means, showing a heterotetrameric complex composed of three A and one B subunits (Shammat and Gordon, 1999; Zheng et al., 2002). Naturally occurring mutations in the cone channel have been shown to affect cone function and to associate with varieties of cone diseases (Kaupp and Seifert, 2002). Unlike the rod channel, the biochemical properties and functional regulation of the cone CNG channel are much less understood; only recently has the molecular identity of the channel B subunit been revealed (Gerstner et al., 2000).
KeywordsMouse Retina Cone Photoreceptor 661W Cell Interphotoreceptor Retinoid Binding Protein Cone Channel
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