Immune Cell Redistribution After Vascularized Bone Marrow Transplantation

  • Waldemar L. Olszewski
  • Marek Durlik

The specificity of a limb transplant lies in its anatomy. It is a graft of an organ, in analogy to the kidney or heart transplant, but in addition, it is a graft of bone marrow (BM) whose cells (BMC) do not only proliferate and mature in the graft but also migrate to the recipient bone marrow cavities and lymphoid organs (LO). Thus, the immune contact between the donor and recipient takes place not only in the graft itself but also in the recipient's immune organs, where the released BMC home. The BMC released from the graft may also home in other tissues, especially those with direct contact with environment as skin, gut, and lungs. Interestingly, there have been reports that the donor bone marrow cells may survive in the allogeneic recipient and create a state of cellular microchimerism. Microchimerisms are, according to some researchers, linked with the development of partial tolerance to donor alloantigens. This is a controversial issue with a number of observations supporting and negating the concept of microchimerism as a causative factor in prolongation of survival of organ allograft transplanted concomitantly with bone marrow. Another problem is the graft-versus-host reaction developing after bone marrow transplantation. There are reports providing evidence for lack of the graft-versus-host reaction after limb transplantation.


Lymphoid Organ Mesenteric Lymph Node Bone Marrow Cavity Recipient Tissue Mesenteric Lymph Node Cell 
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  1. 1.
    T.E. Starzl, A.J. Demetris, N. Murase, S. Ildstad, C. Ricordi, and M. Trucco, Cell migration, chimerism, and graft acceptance, Lancet 339, 1579–1582 (1992).PubMedCrossRefGoogle Scholar
  2. 2.
    J. Kanitakis, D. Jullien, A. Claudy, J.P. Revillard, and J.M. Dubernard, Microchimerism in a human hand allograft, Lancet 354, 1820–1821 (1999).PubMedCrossRefGoogle Scholar
  3. 3.
    C.W. Hewitt, R. Ramsamooj, M.P. Patel, B. Yazdi, B.M. Achauer, and K.S. Black, Development of stable mixed T cell chimerism and transplantation tolerance without immune modulation in recipients of vascularized bone marrow allografts, Transplantation 50, 766–772 (1990).PubMedCrossRefGoogle Scholar
  4. 4.
    R.D. Foster, L. Fan, M. Niepp, et al., Mixed allogeneic chimeras as a model for tolerance induction in composite tissue allografts: chimeric stability and functional recovery, Surg. Forum 52, 605 (2001).Google Scholar
  5. 5.
    M. Vossen, V. Gorantla, G. Perez-Abadia, et al., Vascularized bone marrow transplantation: fact or fiction? Surg. Forum 525, (2001).Google Scholar
  6. 6.
    A. Umemura, H. Morita, X.C. Li, S. Tahan, A.P. Monaco, and T. Maki, Dissociation of hemopoietic chimerism and allograft tolerance after allogeneic bone marrow transplantation, J. Immunol. 167, 3043–3048 (2001).PubMedGoogle Scholar
  7. 7.
    R. Llull, N. Murase, Q. Ye, R. Manez, A.J. Demetris, V. Fournier, and T.E. Starzl, Vascularized bone marrow transplantation in rats: evidence for amplification of hematolymphoid chimerism and freedom from graft-versus-host reaction, Transpl. Proc. 27(1), 164–165 (1995).Google Scholar
  8. 8.
    R. Llull, N. Murase, A.J. Demetris, Q. Ye, R. Manez, and T.E. Starzl, Multilineage ampliafication of graft-vs-host disease-resistant chimerism following rat vascularized bone marrow allotransplantation, Transpl. Proc. 27(4), 2363–2364 (1995).Google Scholar
  9. 9.
    R. Ramsamooj, R. Llull, L.D. Tatem, K.S. Black, V. Lotano, R.M. Dalsey, C.T. Born, W.G. DeLong, and C.W. Hewitt, Graft-versus-host disease in limb transplantation: digital image analysis of bone marrow and TGF-β expression in situ using a novel 3-D microscope, Transpl. Proc. 28, 2029–2031 (1996).Google Scholar
  10. 10.
    V. Gorantla, G. Perez-Abadia, K. Prabhune, H. Orhun, R. Majzoub, T. Kakoulidis, C. Maldonado, G. Anderson, L. Ogden, W. Breidenbach, S. Ildstad, and J. Barker, Composite tissue allografts: tolerance induction without graft-versus-host disease, Plast. Surg. 51, 578–579 (2000).Google Scholar
  11. 11.
    S.P. Hiotis, K.L. Wnuk, W.A. Blumenthals, S.A. Halaris, and R.A. Good, Successful limb transplantation across a multi-minor barrier facilitated by preceding engraftment of T-cell-purged donor and recipient bone marrow, Transpl. Proc. 31, 692–693 (1999).CrossRefGoogle Scholar
  12. 12.
    P.C. Brouha, G. Perez-Abadia, C.G. Francois, V. Gorantla, M. Vossen, L.A. Laurentin-Perez, C. Maldonado, G. Anderson, M. Kon, and J.H. Barker, Prevention of graft-versus-host disease in chimeric hosts by lymph node removal prior to limb allotransplantation, Surg. Forum 52, 527 (2001).Google Scholar
  13. 13.
    M. Durlik, B. Lukomska, A. Namyslowski, E. Cybulska, S. Janczewska, and W.L. Olszewski, The kinetics of seeding of syngeneic cells from vascularized bone marrow grafts, Transpl. Proc. 29, 2008–2009 (1997).CrossRefGoogle Scholar
  14. 14.
    W.L. Olszewski, B. Lukomska, M. Durlik, and A. Ziembinski, Bone marrow in bone versus bone marrow cell suspension transplant, Transpl. Proc. 24(6), 3002–3003 (1992).Google Scholar
  15. 15.
    M. Durlik, B. Lukomska, M. Morzycka-Michalik, and W.L. Olszewski, Bone marrow reconstitution in irradiated rats receiving syngeneic hind limb graft, Eur. Sur. Res. 23(Suppl 1), 30 (1991).Google Scholar
  16. 16.
    B. Lukomska, M. Durlik, M. Morzycka-Michalik, and W.L. Olszewski, Transplantation of vascularized bone marrow, Transpl. Proc. 23(1), 887–888 (1991).Google Scholar
  17. 17.
    W.L. Olszewski, B. Lukomska, M. Durlik, A. Namyslowski, and E. Cybulska, Bone marrow cells transplanted in suspension or in vascularized bone graft repopulate not only bone marrow but also lymphoid organs, Transpl. Proc. 26(6), 3319–3320 (1994).Google Scholar
  18. 18.
    S. Janczewska, A. Ziolkowska, M. Durlik, W.L. Olszewski, and B. Lukomska, Fast lymphoid reconstitution after vascularized bone marrow transplantation in lethally irradiated rats, Transplantation 68(2), 201–209 (1999).PubMedCrossRefGoogle Scholar
  19. 19.
    B. Lukomska, S. Janczewska, M. Durlik, and W.L. Olszewski, Kinetics of bone marrow repopulation in lethally irradiated rats after transplantation of vascularized bone marrow in syngeneic hind limb, Ann. Transpl. 5(1), 14–20 (2000).Google Scholar
  20. 20.
    B. Lukomska, M. Durlik, E. Cybulska, and W.L. Olszewski, Comparative analysis of immunological reconstitution induced by vascularized bone marrow versus bone marrow cell transplantation, Transpl. Int. 9, S492–S496 (1996).PubMedCrossRefGoogle Scholar
  21. 21.
    W.L. Olszewski, B. Lukomska, M. Durlik, A. Namyslowski, D. Laszuk, and E. Cybulska, Nonspecific rapid elimination of transplanted allogeneic bone marrow cells, Transpl. Proc. 29, 2073–2074 (1997).CrossRefGoogle Scholar
  22. 22.
    M. Durlik, B. Lukomska, A. Namyslowski, S. Janczewska, E. Cybulska, and W.L. Olszewski, Long-term survival of limb allografts after weaning of cyclosporine: possible role of microchimerism, Transpl. Proc. 29, 1226–1227 (1997).CrossRefGoogle Scholar
  23. 23.
    M. Durlik, B. Lukomska, P. Religa, H. Ziolkowska, A. Namyslowski, S. Janczewska, E. Cybulska, J. Soin, Z. Gaciong, and W.L. Olszewski, Tolerance induction following allogeneic vescularized bone marrow transplantation – the possible role of microchimerism, Transpl. Int. 11, S229–S302 (1998).Google Scholar
  24. 24.
    W.L. Olszewski, M. Durlik, B. Lukomska, P. Religa, H. Ziolkowska, S. Janczewska, E. Cybulska, J. Soin, Z. Gaciong, and B. Interewicz, Donor DNA can be detected in recipient tissues during rejection of allograft, Transpl. Int. 13, S461–S464 (2000).PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Waldemar L. Olszewski
    • 1
  • Marek Durlik
    • 1
  1. 1.Polish Academy of Sciences Department of Surgical Research and TransplantologyMedical Research Center, Central Clinical Hospital, Ministry of Internal AffairesWarsawPoland

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