Animal and Cell Culture Models to Study Foodborne Pathogen Interaction
Our knowledge of pathogenic mechanism of foodborne pathogens has stemmed from the use of various cultured cell lines and animal models. In early days, animal models were often used to confirm the pathogenic nature of an isolate that was involved in a disease and also to study immune responses. In recent years, however, animals are used as a model to study pathogenic mechanism of a microorganism, immune response to an infection, and to study the efficacy of a vaccine. However, cultured cell lines are now considered indispensable powerful tools in studying the molecular and cellular mechanism of pathogenesis. Both animal and cell culture models are essential for studying pathogenesis, thus one must be familiar with those models and their applications. To study bacterium and host interactions, it is important to use the strain that cause the disease because of the clonal nature of the pathogens. Pathogens may also loose virulence traits during subculture, therefore one has to avoid multiple subculturing (passage) before pathogenicity testing. During pathogenicity testing, some strains may not exhibit pathognomonic symptoms in either model. Therefore, finding a suitable and sensitive animal or cell culture system is crucial for studying host parasite interactions. Organ cultures such as ligated-ileal loop or embryonated-egg model have been used as a substitute for animal models.
KeywordsLethal Dose Cytopathic Effect Cell Culture Model Culture Cell Line Foodborne Pathogen
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