The natural fermentation of Sausages is a complex microbial process in which the main participants are represented by lactic acid bacteria (LAB) and coagulase-negative cocci (CNC). The microflora of different types of fermented Sausages has been defined by using traditional microbiological methods based on isolation and biochemical identification. Since it appears that the types of microbial groups, or even the specific strains of a given microbial group, that dominate the fermentation, significantly affect the organoleptic profile of the final product, there is an increasing interest in the description of the microbiota that are found in different fermented Sausages. Recently new tools, based on molecular methods, allowing fast and unequivocal identification of strains, isolated from fermented Sausages, became available. These methods have been successfully applied and, in general, biochemical and molecular identification compared well. However, new information comes to light when molecular methods are applied to DNA and/or RNA extracted directly from Sausages. This approach eliminates problems related to traditional isolation. This chapter deals with the recent findings and results of the application of molecular methods, in a culture-dependent and culture-independent manner, on the study of the microflora of fermented Sausages.
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© 2008 Springer Science+Business Media, LLC
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Rantsiou, K., Cocolin, L. (2008). Fermented Meat Products. In: Cocolin, L., Ercolini, D. (eds) Molecular Techniques in the Microbial Ecology of Fermented Foods. Food Microbiology and Food Safety. Springer, New York, NY. https://doi.org/10.1007/978-0-387-74520-6_3
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DOI: https://doi.org/10.1007/978-0-387-74520-6_3
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-74519-0
Online ISBN: 978-0-387-74520-6
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