Milk Fat Globule Membrane Components–A Proteomic Approach

  • Maria Cavaletto
  • Maria Gabriella Giuffrida
  • Amedeo Conti
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 606)


The milk fat globule membrane (MFGM) is the membrane surrounding lipid droplets during their secretion in the alveolar lumen of the lactating mammary gland. MFGM proteins represent only 1–4% of total milk protein content; nevertheless, the MFGM consists of a complex system of integral and peripheral proteins, enzymes, and lipids. Despite their low classical nutritional value, MFGMproteins have been reported to play an important role in various cellular processes and defense mechanisms in the newborn.

Using a proteomic approach, such as high-resolution, two-dimensional electrophoresis followed by direct protein identification by mass spectrometry, it has been possible to comprehensively characterize the subcellular organization of MFGM.

This chapter covers the description of MFGM proteomics from the first studies about 10 years ago through the most recent papers. Most of the investigations deal with MFGMs from human and cow milk.


Lipid Droplet Xanthine Oxidase Proteomic Approach Peptide Mass Fingerprinting Lipid Globule 


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Maria Cavaletto
    • 1
  • Maria Gabriella Giuffrida
  • Amedeo Conti
  1. 1.Biochemistry and Proteomics Section, DISAV Dipartimento Scienze dell’ Ambiente e della VitaUniversità del Piemonte OrientaleAlessandriaItaly

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