Combinatorial Libraries of Arrayable Single-Chain Antibodies

  • Itai Benhar
Part of the Integrated Analytical Systems book series (ANASYS)


Antibodies that bind their respective targets with high affinity and specificity have proven to be essential reagents for biological research. Antibody phage display has become the leading tool for the rapid isolation of single-chain variable fragment (scFv) antibodies in vitro for research applications, but there is usually a gap between scFv isolation and its application in an array format suitable for high-throughput proteomics. In this chapter, we present our antibody phage display system where antibody isolation and scFv immobilization are facilitated by the design of the phagemid vector used as platform. In our system, the scFvs are fused at their C-termini to a cellulose-binding domain (CBD) and can be immobilized onto cellulose-based filters. This made it possible to develop a unique filter lift screen that allowed the efficient screen for multiple binding specificities, and to directly apply library-derived scFvs in an antibody spotted microarray.


scFv Antibody Antibody Library Antibody Domain Generic Ligand Affinity Selection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Acknowledgments and Notes

The work reported in this chapter was supported in part by a research grant from the Israel Science Foundation, administered by the Israel National Academy for Sciences and Humanities (Jerusalem, Israel). The n-CoDeR® technology is covered by IP rights held by BioInvent, Sweden (


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Copyright information

© Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Itai Benhar
    • 1
  1. 1.Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life SciencesTel-Aviv UniversityIsrael

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