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DNAzymes in DNA Nanomachines and DNA Analysis

  • Yu He
  • Ye Tian
  • Yi Chen
  • Chengde Mao
Part of the Integrated Analytical Systems book series (ANASYS)

Abstract

This chapter discusses our efforts in using DNAzymes in DNA nano-machines and DNA analysis systems. 10–23 DNAzymes can cleave specific phos-phodiester bonds in RNA. We use them to construct an autonomous DNA-RNA chimera nanomotor, which constantly extracts chemical energy from RNA substrates and transduces the energy into a mechanical motion: cycles of contraction and extension. The motor's motion can be reversibly turned on and off by a DNA analogue (brake) of the RNA substrate. Addition and removal of the brake stops and restarts, respectively, the motor's motion. Furthermore, when the RNA substrates are preorganized into a one-dimensional track, a DNAzyme can continuously move along the track so long as there are substrates available ahead. Based on a similar mechanism, a novel DNA detection system has been developed. A target DNA activates a DNAzyme to cleave RNA-containing molecular beacons (MB), which generates an enhanced fluorescence signal. A following work integrates two steps of signal amplifications: a rolling-circle amplification (RCA) to synthesize multiple copies of DNAzymes, and the DNAzymes catalyze a chemical reaction to generate a colorimetric signal. This method allows detection of DNA analytes whose concentration is as low as 1 pM.

Keywords

Signal Amplification Mechanical Motion Molecular Beacon Nanomechanical Device Inhibitory Segment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work is supported by the NSF (EIA-0323452) and Purdue University (a start-up fund).

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Yu He
  • Ye Tian
  • Yi Chen
  • Chengde Mao

There are no affiliations available

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