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Nucleic Acids for Computation

  • Joanne Macdonald
  • Milan N. Stojanovic
Part of the Integrated Analytical Systems book series (ANASYS)

Abstract

Nucleic acids have many features that are ideal for molecular computation. Using nucleic acids, we have constructed a full set of molecular logic gates, with modular stem-loop-controlled deoxyribozymes as switches and single-stranded oligonucleotides as inputs and outputs. These gates have been combined to form basic computational circuits, including a half- and a full-adder, and can also be assembled into automata to perform complex computational tasks such as game playing. Our most advanced automaton to-date integrates more than 100 nucleic acid logic gates to play a complete game of tic-tac-toe encompassing 76 possible game plays. Inputs and outputs can also be coupled with upstream and downstream components, such as aptamers, sensors, secondary gate activation, and small-molecule release, indicating the potential for nucleic acid computation in the engineering of autonomous therapeutic and diagnostic molecular devices.

Keywords

Human Move Logic Gate Molecular Computation Human Player Input Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This material is based upon work supported by the National Science Foundation under Grants IIS-0324845, CCF-0523317, and CHE-0533065, Searle Fellowship to M.N.S. Milan Stojanovic is a Lymphoma and Leukemia Society Fellow.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Joanne Macdonald
    • 1
  • Milan N. Stojanovic
    • 1
  1. 1.The National Science Foundation Center for Molecular Cybernetics; Division of Experimental Therapeutics, Department of MedicineColumbia UniversityNew YorkUSA

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