Catecholamine neurons (CA) located in the brainstem project widely in the forebrain, hindbrain and spinal cord to many regions involved in the control of respiratory and cardiovascular function. For example, A6 noradrenergic neurons provide a tonic excitatory stimulus that maintains breathing frequency while A5 neurons provide an inhibitory influence on both cardiovascular and respiratory function to slow breathing frequency and heart rate. Mice with genetic defects that include CA neurons have abnormal respiration and blood pressure. For example, mice heterozygous for Phox2b with CA neuron defects have sleep-disordered breathing, and DBH knockout mice with absent CA cells centrally and peripherally have hypotension. Our hypothesis is that widespread brainstem CA neuron lesions in adult rats would significantly affect cardiorespiratory functions including breathing, chemoreception, blood pressure and heart rate. We produced the widespread brainstem CA neuron lesion by injecting anti-dopamine-β-hydroxylase-saporin (DBH-SAP) via the 4th ventricle. The lesioned group had a 64–84% loss of A5, A6 and A7 tyrosine hydroxylase immunoreactive (ir) neurons along with 56–64% loss of C1 and C2 phenyl ethanolamine-N methyltransferase (PNMT)-ir neurons over 2–3 weeks. The significant respiratory changes included: (1) a decreased breathing frequency during air and 7% CO2 breathing in both wakefulness and non-REM (NREM) sleep; (2) a reduced ventilatory response to 7% CO2 in wakefulness (−28%) and in NREM sleep (−26%); and (3) increased variability of breathing in REM sleep but not in wakefulness or NREM sleep. Significant cardiovascular changes at two weeks included: (1) an increased MAP by 11.7 mmHg in the room air resting condition; (2) an unaffected cardiovascular response to hypercapnia; and (3) a smaller decrease of MAP in response to hypoxia.Conclusions: Central CA neurons have a net excitatory effect on breathing and chemoreception but a net inhibitory effect on blood pressure.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Carson, R. and Robertson, D. (2002) Genetic manipulation of noradrenergic neurons. J. Pharmacol. Exp. Ther. 301, 410–417.
Del Bo, A., Le Doux, J. and Resi, D. (1985) Sympathetic nervous system and control of blood pressure during natural behaviour. J. Hypertens. Supp. 3, S105–S106.
Erickson, J., Conover, J., Borday, V., Champagnat, J., Barbacid, M., Yancopoulos, G. and Katz, D. (1996) Mice lacking brain-derived neurotrophic factor exhibit visceral sensory neuron losses distinct from mice lacking NT4 and display a severe developmental deficit in control of breathing. J. Neurosci. 16, 5361–5371.
Guyenet, P., Koshiya, N., Huangfu, D., Verberne, A. and Riley, T. (1993) Central respiratory control of A5 and A6 pontine noradrenergic neurons. Am. J. Physiol. 264, 1035–1044.
Hilaire, G. (2006) Endogenous noradrenaline affects the maturation and function of the respiratory network: possible implication for SIDS. Auton. Neurosci. 126–127, 320–331.
Ide, S., Itoh, M. and Goto, Y. (2005) Defect in normal developmental increase of the brain biogenic amine concentrations in the mecp2-null mouse. Neurosci. Lett. 386, 14–17.
Janssen, B. and Smits, J. (2002) Autonomic control of blood pressure in mice: basic physiology and effects of genetic modification. Am. J. Physiol. Regul. Integr. Comp. Physiol. 282, R1545–1564.
Li, A. and Nattie, E. (2006) Catecholamine neurones in rats modulate sleep, breathing, central chemoreception and breathing variability. J. Physiol. 570, 385–396.
Schreihofer, A. and Guyenet, P. (2000) Sympathetic reflexes after depletion of bulbospinal catecholaminergic neurons with anti-DBH-saporin. Am. J. Physiol. Regul. Integr. Comp. Physiol. 279, R729–R742.
Shirasawa, S., Arata, A., Onimaru, H., Roth, K., Brown, G., Horning, S., Arata, S., Okumura, K., Sasazuki, T. and Korsmeyer, S. (2000) Rnx deficiency results in congenital central hypoventilation. Nat. Genet. 24, 287–290.
Swoap, S., Weinshenker, D., Palmiter, R. and Garber, G. (2004) Dbh(-/-) mice are hypotensive, have altered circadian rhythms and have abnormal responses to dieting and stress. Am. J. Physiol. Regul. Integr. Comp. Physiol. 286, R108–113.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer
About this chapter
Cite this chapter
Li, A., Emond, L., Nattie, E. (2008). Brainstem Catecholaminergic Neurons Modulate both Respiratory and Cardiovascular Function. In: Poulin, M.J., Wilson, R.J.A. (eds) Integration in Respiratory Control. Advances in Experimental Medicine and Biology, vol 605. Springer, New York, NY. https://doi.org/10.1007/978-0-387-73693-8_65
Download citation
DOI: https://doi.org/10.1007/978-0-387-73693-8_65
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-73692-1
Online ISBN: 978-0-387-73693-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)