A Novel Hydrogel Functionalized with Specific Peptidomimetic Ligands for 2-D and 3-D Cell Culture
It is believed that there is a close relationship between the three-dimensional (3-D) architecture of the cells and their differentiation. The morphologic cell integrity in the 3-D cell culture as well as the numerous cell—matrix and cell—cell interactions promotes intercellular communications, which favors the proper biochemical regulation of the tumor cell in vivo. Various 2-D and 3-D supports based on natural or synthetic materials have been used for the cell culture. PVA is a promising biomaterial accepted by food and drug organizations (e.g., FDA) in various countries, and widely used for biomedical applications such as artificial blood vessels, synthetic vitreous, and implantable medical materials because of its bioinertness. PVA is ready to be functionalized and PVA aqueous solution below 15% remains to be liquid at room temperature which is feasible for the conjugation of biomolecules onto PVA matrix. Agarose is a natural biomaterial which forms gel in water at...
KeywordsJurkat Cell Hybrid Hydrogel Stable Gelation Easy Modification Artificial Blood Vessel
The financial supports from NSF CH 0302122; NIH/NCI NCDDG U19 CA113298; NIH/NCI Ro1CA115483 are greatly acknowledged.