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Expression and Characterization of Recombinant S2 Subunit of SARS-coronavirus S Fusion Protein

  • Zhe Yan
  • Kathryn V. Holmes
  • Robert S. Hodges
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

The SARS-CoV Spike fusion protein subunit S2 plays an important role in viral entry by initiating fusion of the viral and cellular membranes. S2 exists in at least three different conformations within the trimeric glycoprotein complex. In the postfusion conformation (six-helix bundle, 6HB), the three helical heptad repeats of the HRC region pack in an antiparallel fashion into the hydrophobic grooves on the surface of the trimeric coiled-coil formed by the heptad repeat N-terminal (HRN) region. The HRN and HRC regions are separated by a 149 amino acid interhelical domain (IHD), which is thought to provide the flexibility required for the conformational change that occurs during the membrane fusion process. There is no structural information currently available for the interhelical domain and S2 in the prefusion state and fusion intermediate states.

In this study, we have expressed the recombinant S2 subunit of SARS-CoV S fusion protein including the interhelical domain and...

Keywords

Fusion Protein Heptad Repeat Hydrophobic Groove Amylose Resin Membrane Fusion Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by a NIH grant (PO1AI059576) and the John Stewart Chair in Peptide Chemistry to R. S. H.

References

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Zhe Yan
    • 1
  • Kathryn V. Holmes
    • 2
  • Robert S. Hodges
    • 1
  1. 1.Department of Biochemistry and Molecular GeneticsUniversity of Colorado at Denver and Health Sciences CenterAuroraUSA
  2. 2.Department of MicrobiologyUniversity of Colorado at Denver and Health Sciences CenterAuroraUSA

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