Effects of Antifreeze Protein Fragments on the Properties of Model Membranes
During the past three decades a considerable amount of research has been conducted on the characterization of and structure — activity studies of antifreeze proteins. The ability of antifreeze proteins to protect cell membranes from hypothermic damage was first demonstrated by Rubinsky et al. . In addition antifreeze proteins have been shown to prevent leakage of liposomes as they were cooled through their phase transition temperatures . These results suggested interactions of antifreeze proteins with the lipid bilayer of the cell membrane. One cause of cold induced cellular damage could arise when cells are cooled through the lipid membrane thermotropic phase transition temperature. During this liquid crystalline to gel phase transition, membranes become leaky, resulting in the loss of intracellular contents and an influx of extracellular materials. Evidence consistent with this hypothesis comes from studies on human blood platelets where it was demonstrated that antifreeze...
KeywordsDifferential Scanning Calorimetry Phase Transition Temperature Short Segment Membrane Stabilization Isothermal Titration Calorimetry
This research was supported by the Israel Science Foundation (ISF) grant No. 289.
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