Advertisement

Unique Role of Clusters of Electrostatic Attractions in Controlling the Stability of Two-stranded α-Helical Coiled-Coils

  • J. Paul Kirwan
  • Stan C. Kwok
  • Susanna McReynolds
  • Janine Mills
  • David Osguthorpe
  • Robert S. Hodges
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

A major goal of protein research is to relate amino acid sequence to protein structure and function. Describing all inter-residue interactions that contribute to the threshold stability required to initiate protein folding is key to achieving this end. The two-stranded α-helical coiled-coil is a simplified protein motif well suited for studying these interactions. Our laboratory has shown that clusters of stabilizing and destabilizing residues in the hydrophobic core control coiled-coil stability [1, 2]. We have also shown that the effect of chain length on coiled-coil stability depends on the composition of the added/removed heptads [3]. Here we investigate the existence of stabilizing sequence electrostatic-clusters and whether they trigger folding and confer final stability, or confer final stability without governing folding.

Results and Discussion

We prepared a series of C-terminal deletion fragments of the 284 residue coiled-coil tropomyosin (1–81, 1–92, 1–99, 1–119,...

Keywords

Polypeptide Chain Hydrophobic Core Final Stability Native Sequence Alanine Residue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Kwok, S.C. and Hodges, R.S., J Biol. Chem. 278, 35248–35254 (2003).CrossRefGoogle Scholar
  2. 2.
    Kwok, S.C. and Hodges, R.S., J Biol. Chem. 279, 21576–21588 (2004).CrossRefGoogle Scholar
  3. 3.
    Kwok, S.C. and Hodges, R.S., J. Biopolymers. 76, 378–390 (2004).CrossRefGoogle Scholar
  4. 4.
    Wagschal, K., Tripet, B., and Hodges, R.S., J. Mol. Biol. 285, 785–803 (1999).CrossRefGoogle Scholar
  5. 5.
    Wagschal, K., Tripet, B., Lavigne, P., Mant, C.T. and Hodges, R.S., Protein Sci. 11, 2312–2329 (1999).Google Scholar
  6. 6.
    Tripet, B., Wagschal, K., Lavigne, P., Mant, C.T. and Hodges, R.S., J. Mol. Biol. 300, 377–402 (2000).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • J. Paul Kirwan
    • 1
  • Stan C. Kwok
    • 1
  • Susanna McReynolds
    • 1
    • 2
  • Janine Mills
    • 1
  • David Osguthorpe
    • 3
  • Robert S. Hodges
    • 1
  1. 1.Department of Biochemistry & Molecular GeneticsUniversity of Colorado at Denver and Health Sciences CenterAuroraUSA
  2. 2.Department of MicrobiologyUniversity of Colorado at Denver and Health Sciences CenterAuroraUSA
  3. 3.Department of PharmacologyUniversity of Colorado at Denver and Health Sciences CenterAuroraUSA

Personalised recommendations