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The role of Cell Penetrating Peptides (CPPs) in membrane lipid phase behavior: a novel aspect elucidating peptide-mediated delivery

  • Cinzia Esposito
  • AiWei Tian
  • Mario Scrima
  • Karolina Kieres
  • Pietro Campiglia
  • Anna Maria D'Ursi
  • Tobias Baumgart
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

Cell Penetrating Peptides (CPPs) constitute a class of short cationic peptides, which share the ability to deliver therapeutic agents (proteins, oligonucleotides) into target cells. The mechanisms proposed for the CPPs cellular uptake (endocytic, endosomal escape, etc.) are still controversial.

In the hypothesis that membrane lateral heterogeneity generated by multi lipid and protein components in cell membrane could play an important role in CPPs translocation process, we conducted studies on two popular CPPs, penetratin (RQIKIWFQNRRMKWKK) [1] and pep-1 (KETWWETWWTEWSQPKKKRKV) [2], in the presence of giant unilamellar vesicles (GUVs). In particular, we used GUVs containing different quaternary mixtures of 1,2-Dioleoyl-sn-Glycero-3-Phosphocholine (DOPC), 1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine (DPPC), Cholesterol (Chol), 1,2-Dipalmitoyl-sn-Glycero-3-[Phospho-rac-(1-glycerol)] (Sodium Salt) (DPPG) and showing liquid-liquid fluid phase coexistence, which provides an...

Keywords

Lipid Raft Phase Coexistence Endosomal Escape Translocation Process Giant Unilamellar Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Thoren Per E.G., et al. FEBS Letters 482, 265–268 (2000).CrossRefGoogle Scholar
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    Henriques S.T., et al. Biochim Biophys Acta. 1669, 75–86 (2005).CrossRefGoogle Scholar
  3. 3.
    Baumgart, T., et al. Nature 425, 821–824 (2003).CrossRefGoogle Scholar
  4. 4.
    Baumgart, T., et al. Proc Natl Acad Sci USA. 9, 3165–3170 (2007)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Cinzia Esposito
    • 1
    • 2
  • AiWei Tian
    • 1
  • Mario Scrima
    • 2
  • Karolina Kieres
    • 2
  • Pietro Campiglia
    • 2
  • Anna Maria D'Ursi
    • 1
  • Tobias Baumgart
    • 2
  1. 1.Department of ChemistryUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Dipartimento di Scienze FarmaceuticheUniversity of SalernoFiscianoItaly

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