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Spatially Defined Peptide-Polymer Biotherapeutic Synthesis on Novel Cross-linked Beads with ‘Spatially Tunable’ and ‘Isolated’ Functional Sites

  • M.K. Jacob
  • K.S. Kumar
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

Functionalized crosslinked polymers prepared by either pre or post functionalization strategies do not have any control over the point of functional group incorporation within the polymer network. Synthesis of polypeptide using these polymers can lead to the formation of number of deletion and truncated peptide sequences due to unfavorable site-site interaction between the growing peptide chains and also due to macromolecular network of the polymer. The subsequent conjugation with high molecular weight linear polymers can results in difficulties during purification of the target polymer-bioconjugate. This paper discusses about the synthesis and application of a novel crosslinked polymer in which unfavorable site-site interactions are avoided by the spatial tuning of functional sites to a predefined distance within cross-linked polymers. In addition to ‘site isolation’ the polymer has been designed in such a way that all functional sites have a homogeneous and amphipathic...

Keywords

Antimicrobial Peptide Functional Site Suspension Polymerization Hydroxyl Functional Group Spatial Tuning 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank CSIR, New Delhi for the project funding to KSK & research fellowship to MKJ.

References

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    Delgado, M., Janda, K.D. Current Organic Chemistry. 6, 1031–1043 (2002).CrossRefGoogle Scholar
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    Fields, G. B. Methods Enzymol. 289, 3–13 (1999).Google Scholar
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    Yi-An Lu. Arthur, M. F. Reactive Polymers. 22, 221–229 (1994).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • M.K. Jacob
    • 1
  • K.S. Kumar
    • 1
  1. 1.Division of Molecular MedicineRajiv Gandhi Centre for BiotechnologyThiruvananthapuramIndia

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