Spatially Defined Peptide-Polymer Biotherapeutic Synthesis on Novel Cross-linked Beads with ‘Spatially Tunable’ and ‘Isolated’ Functional Sites
Functionalized crosslinked polymers prepared by either pre or post functionalization strategies do not have any control over the point of functional group incorporation within the polymer network. Synthesis of polypeptide using these polymers can lead to the formation of number of deletion and truncated peptide sequences due to unfavorable site-site interaction between the growing peptide chains and also due to macromolecular network of the polymer. The subsequent conjugation with high molecular weight linear polymers can results in difficulties during purification of the target polymer-bioconjugate. This paper discusses about the synthesis and application of a novel crosslinked polymer in which unfavorable site-site interactions are avoided by the spatial tuning of functional sites to a predefined distance within cross-linked polymers. In addition to ‘site isolation’ the polymer has been designed in such a way that all functional sites have a homogeneous and amphipathic...
KeywordsAntimicrobial Peptide Functional Site Suspension Polymerization Hydroxyl Functional Group Spatial Tuning
The authors thank CSIR, New Delhi for the project funding to KSK & research fellowship to MKJ.