Advertisement

Effects of net charge and the number of positively charged residues on the biological activity of amphipathic α-helical cationic antimicrobial peptides

  • Ziqing Jiang
  • Adriana I. Vasil
  • John Hale
  • Robert E. W. Hancock
  • Michael L. Vasil
  • Robert S. Hodges
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

The widespread use of classical antibiotics has resulted in the emergence of many antibiotic-resistant strains. Cationic antimicrobial peptides (AMPs) have become important candidates as potential therapeutic agents. Cationic AMPs of the α-helical class have two unique features: a net positive charge of at least +2 and an amphipathic character, with a non-polar face and a polar/charged face.

We previously designed a peptide V13K from our original α-helical antimicrobial peptide V681, which had excellent antimicrobial activity but also exhibited high toxicity [1]. D-V13K showed greater antimicrobial activity, no toxicity and excellent stability to proteolytic digestion compared to D-V681. The valine to lysine substitution in the center of the non-polar face is the first report of a specificity determinant in α-helical antimicrobial peptides between eukaryotic and prokaryotic cells. The hydrophobic residues on the non-polar face of the helix played a more important role in...

Keywords

Antimicrobial Activity Therapeutic Index Hemolytic Activity Minimal Inhibitory Concentration Cationic Antimicrobial Peptide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Chen, Y., C. T. Mant, S. W. Farmer, R. E. Hancock, M. L. Vasil and R. S. Hodges. J Biol Chem 280 (13), 12316–12329 (2005).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Ziqing Jiang
    • 1
  • Adriana I. Vasil
    • 2
  • John Hale
    • 3
  • Robert E. W. Hancock
    • 3
  • Michael L. Vasil
    • 2
  • Robert S. Hodges
    • 1
  1. 1.Department of Biochemistry & Molecular GeneticsUniversity of Colorado at Denver and Health Sciences CenterAuroraUSA.
  2. 2.Department of MicrobiologyUniversity of Colorado at Denver and Health Sciences CenterAuroraUSA.
  3. 3.Department of Microbiology and ImmunologyUniversity of British ColumbiaVancouverCanada

Personalised recommendations