Fallaxin analogues with improved antibacterial activity
The antibacterial peptide ”fallaxin” H-GVVDILKGAAKDIAGHLASKVMNKL-NH2, recently isolated from the West-Indian mountain chicken frog ”leptodactylus fallax”, have been shown to inhibit the growth of a number of Gram-negative bacteria including E.coli, P.aeruginosa and K.pneumoniae . Antibacterial activity and selectivity has in studies shown to depend on various parameters i.e. the net charge, mean hydrophobicity <H>, mean hydrophobic moment <μH> and the ability to fold into amphipatic structures upon interaction with membrane models . To gain further insight into the structure-activity relationship of fallaxin, we performed a complete alanine-scan of fallaxin. The peptides were tested for antibacterial activity against methicilin-resistant S.aureus (MRSA), vancomycin-intermediate resistant S.aureus, (VISA), E.coli and K.pneumoniae. Finally, the cytotoxicity of the fallaxin analogues against human erythrocytes was assessed in a hemolytic activity assay
KeywordsAntibacterial Activity Human Erythrocyte Activity Profile Peptide Synthesis Hemolytic Activity
SLN gratefully acknowledges a scholarship from Novozymes/Novo-Nordisk.