Fallaxin analogues with improved antibacterial activity

  • S. L. Nielsen
  • N. Frimodt-Møller
  • P. R. Hansen
Part of the Advances in Experimental Medicine and Biology book series (volume 611)


The antibacterial peptide ”fallaxin” H-GVVDILKGAAKDIAGHLASKVMNKL-NH2, recently isolated from the West-Indian mountain chicken frog ”leptodactylus fallax”, have been shown to inhibit the growth of a number of Gram-negative bacteria including E.coli, P.aeruginosa and K.pneumoniae [1]. Antibacterial activity and selectivity has in studies shown to depend on various parameters i.e. the net charge, mean hydrophobicity <H>, mean hydrophobic moment <μH> and the ability to fold into amphipatic structures upon interaction with membrane models [2]. To gain further insight into the structure-activity relationship of fallaxin, we performed a complete alanine-scan of fallaxin. The peptides were tested for antibacterial activity against methicilin-resistant S.aureus (MRSA), vancomycin-intermediate resistant S.aureus, (VISA), E.coli and K.pneumoniae. Finally, the cytotoxicity of the fallaxin analogues against human erythrocytes was assessed in a hemolytic activity assay

Results and...


Antibacterial Activity Human Erythrocyte Activity Profile Peptide Synthesis Hemolytic Activity 
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SLN gratefully acknowledges a scholarship from Novozymes/Novo-Nordisk.


  1. 1.
    Rollins-Smith, L.A., et al. (2005) An Antimicrobial peptide from the skin secretions of the mountain chicken frog Leptodactulys fallax (Anura: Leptodactylidae) Regulatory Peptides 124: 173–179CrossRefGoogle Scholar
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    Hancock, R.E.W., and Sahl, H.G., (2006). Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nature Biotechnology 24: 1551–1557.CrossRefGoogle Scholar
  3. 3.
    Nielsen, S.L., et al. (2007) Structure-activity study of the antibacterial peptide fallaxin. Protein Science 16: 1969–1976.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • S. L. Nielsen
    • 1
  • N. Frimodt-Møller
    • 2
  • P. R. Hansen
    • 1
  1. 1.Department of Natural Sciences, Faculty of Life SciencesUniversity of CopenhagenDenmark
  2. 2.National Center for Antimicrobials and Infection ControlStatens Serum InstitutDenmark

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