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Antimicrobial Peptides Chelating Lanthanide Ions: the Case of Trichogin GA IV Analogues and Terbium(III)

  • Mariano Venanzi
  • Emanuela Gatto
  • Lorenzo Stella
  • Gianfranco Bocchinfuso
  • Antonio Palleschi
  • Fernando Formaggio
  • Claudio Toniolo
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

Lipopeptaibols are the members of a family of antimicrobial peptides characterized by the presence of a lipid chain at the N-terminus and a high content of α-aminoisobutyric acid (Aib). Trichogin GA IV is one of the most representative compounds of this class, being characterized by peculiar conformational properties and a high affinity to the membrane phase. X-Ray diffraction studies showed that this peptide is folded in a mixed 310/α-helix conformation, while NMR, CD and IR absorption experiments indicated that this structure is also predominantly populated in solution. Recently, we have characterized the aggregation properties and partition equilibria of trichogin GA IV fluorescent analogues in aqueous solution and in the membrane phase, showing that their membrane permeabilization capability is strictly correlated to the concentration of aggregated forms in the lipid phase [1]. We have also recently found that trichogin GA IV shows peculiar binding properties to...

Keywords

Energy Transfer Process Membrane Phase Fluorescent Analogue Fret Efficiency Lanthanide Chelate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was funded by the grants PRIN 2006 (MIUR of Italy) and MAE.

References

  1. 1.
    Mazzuca, C., Stella, L., Venanzi, M., Formaggio, F., Toniolo, C. and Pispisa, B. Biophys. J. 88, 3411–3421 (2005).CrossRefGoogle Scholar
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    Gatto, E., Ph.D. Dissertation, 2007, University of Rome “Tor Vergata”.Google Scholar
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    Gatto, E., Mazzuca, C., Stella, L., Venanzi, M., Toniolo, C. and Pispisa, B. J. Phys. Chem. B 110, 22813–22818 (2006).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Mariano Venanzi
    • 1
  • Emanuela Gatto
    • 1
  • Lorenzo Stella
    • 1
  • Gianfranco Bocchinfuso
    • 1
  • Antonio Palleschi
    • 1
  • Fernando Formaggio
    • 2
  • Claudio Toniolo
    • 2
  1. 1.Department of Chemical Sciences and TechnologiesUniversity of Rome “Tor Vergata”RomeItaly
  2. 2.ICB, Padua Unit, CNR, Department of ChemistryUniversity of PaduaPaduaItaly

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