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Synthesis Of Organometallic Glycopeptides And Electrochemical Studies To Detect Autoantibodies In Multiple Sclerosis Patients'Sera.

  • Feliciana Real-Fernández
  • Amélie Chamois-Colson
  • Jérome Bayardon
  • Francesca Nuti
  • Elisa Peroni
  • Maria R. Moncelli
  • Rita Meunier-Prest
  • Sylvain Jugè
  • Anna Maria Papini
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

Measurement of disease specific biomarkers, such as antibodies, is an important tool for diagnosis, follow-up, and prevention of autoimmune diseases. Identification of autoantibodies is achieved up to now using native protein antigens in immunoenzymatic assays. Unfortunately, in autoimmune diseases only very low specific antibodies are detected in serum, possibly because protein antigens used in the assays contain more than one epitope, of which only few are involved in the disease. Moreover, the use of recombinant protein antigens doesn't allow reproduction of aberrant post-translational modifications (PTMs) involved in triggering autoantibodies. These observations prompted us to set up an innovative “chemical reverse approach” to select specific synthetic peptide antigens containing the minimal epitope with the correct PTM to fishing out specific biomarkers in biological fluids [1].

As a proof-of-concept, we have recently developed CSF114(Glc), a structure-based designed...

Keywords

Cyclic Voltammetry Multiple Sclerosis Patient Gold Electrode Protein Antigen Unnatural Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

PAI Galilée N°11686QA (2006), FIRB Internalization 2005–2008 RBIN04TWKN, MIUR (Italy), Ente Cassa Risparmio di Firenze and 20th APS travel grant committee are gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Feliciana Real-Fernández
    • 1
  • Amélie Chamois-Colson
    • 2
  • Jérome Bayardon
    • 2
  • Francesca Nuti
    • 1
  • Elisa Peroni
    • 1
  • Maria R. Moncelli
  • Rita Meunier-Prest
    • 2
  • Sylvain Jugè
    • 2
  • Anna Maria Papini
    • 1
  1. 1.Laboratory of Peptide & Protein Chemistry & BiologyPolo Scientifico, University of FlorenceSesto FiorentinoItaly
  2. 2.Laboratoire de Synthese et d'Electrosynthese Organometalliques (LSEO)Université de BourgogneDijonFrance

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