Synthesis Of Organometallic Glycopeptides And Electrochemical Studies To Detect Autoantibodies In Multiple Sclerosis Patients'Sera.
Measurement of disease specific biomarkers, such as antibodies, is an important tool for diagnosis, follow-up, and prevention of autoimmune diseases. Identification of autoantibodies is achieved up to now using native protein antigens in immunoenzymatic assays. Unfortunately, in autoimmune diseases only very low specific antibodies are detected in serum, possibly because protein antigens used in the assays contain more than one epitope, of which only few are involved in the disease. Moreover, the use of recombinant protein antigens doesn't allow reproduction of aberrant post-translational modifications (PTMs) involved in triggering autoantibodies. These observations prompted us to set up an innovative “chemical reverse approach” to select specific synthetic peptide antigens containing the minimal epitope with the correct PTM to fishing out specific biomarkers in biological fluids .
As a proof-of-concept, we have recently developed CSF114(Glc), a structure-based designed...
KeywordsCyclic Voltammetry Multiple Sclerosis Patient Gold Electrode Protein Antigen Unnatural Amino Acid
PAI Galilée N°11686QA (2006), FIRB Internalization 2005–2008 RBIN04TWKN, MIUR (Italy), Ente Cassa Risparmio di Firenze and 20th APS travel grant committee are gratefully acknowledged.
- 5.Papini, A.M., Rovero P., Chelli M., Lolli F., Granted U.S.A. Patent & PCT Application WO 03/000733 A2.Google Scholar