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Gadd45β dimerization does not affect MKK7 binding

  • Laura Tornatore
  • Simona M. Monti
  • Daniela Marasco
  • Nina Dathan
  • Rosa M. Vitale
  • Ettore Benedetti
  • Carlo Pedone
  • Salvatore Papa
  • Guido Franzoso
  • Menotti Ruvo
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

The Gadd45 family comprises three small acidic proteins, α, β, and γ, largely involved in cellular response to genotoxic stress and DNA damage. They exert and mediate a large number of functions, including regulation of cell cycle, indeed, it has been demonstrated that, depending on cell type or tissues, Gadd45α and Gadd45γ have strong pro-apoptotic properties, whereas, Gadd45β is a potent mediator of the protective effects of NF-kB against the TNFα induced apoptosis [1,2]. Gadd45β fulfils this function by strongly interacting with MKK7, an essential JNK activator, and blocking its catalytic activity [1,2] and this mechanism has been largely investigated elucidating key residues on both the kinase [1] and the Gadd45 protein [2]. The regions 132–156 of MKK7, containing the ATP-binding K149, and 60–86 of Gadd45β, containing a large acidic stretch, participate in this interaction and the corresponding synthetic peptides strongly block the Gadd45β/MKK interaction and promote...

Keywords

Catalytic Pocket High Order Oligomer Gadd45 Protein Covalent Dimer Unlabelled Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Financial support from project FIRB RBNE03PX83_005 is acknowledged.

References

  1. 1.
    Papa, S., F. Zazzeroni, et al. Nat. Cell. Biol. 6, 146–153 (2004).CrossRefGoogle Scholar
  2. 2.
    Papa, S., S. M. Monti, et al. J. Biol. Chem. 282, 19029–19041 (2007).CrossRefGoogle Scholar
  3. 3.
    Kovalsky, O., F. D. Lung, et al. J. Biol. Chem. 276, 39330–39339 (2001).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Laura Tornatore
    • 1
    • 2
  • Simona M. Monti
    • 1
  • Daniela Marasco
    • 1
  • Nina Dathan
    • 1
  • Rosa M. Vitale
    • 1
  • Ettore Benedetti
    • 1
    • 2
  • Carlo Pedone
    • 1
    • 2
  • Salvatore Papa
    • 3
  • Guido Franzoso
    • 3
  • Menotti Ruvo
    • 1
  1. 1.Istituto di Biostrutture e Bioimmagini — CNR, via MezzocannoneNapoliItaly
  2. 2.Università Federico II di Napoli, via MezzocannoneNapoliItaly
  3. 3.The Ben May Institute for Cancer Research and the University of ChicagoChicago

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