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Molecular Imaging and Orientational Changes of Antimicrobial Peptides in Membranes

  • Tzong-Hsien Lee
  • Kristopher Hall
  • Adam Mechler
  • Lisandra Martin
  • Jonathan Popplewell
  • Gerry Ronan
  • Marie-Isabel Aguilar
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

Resistance to conventional antibiotics has placed antimicrobial peptides under the spotlight as alternative therapeutics for microbial infections. However, the design of specific non-toxic peptides has been elusive due largely to our poor understanding of the precise mechanism of cell lysis. Specifically, the difficulty in defining peptide conformation and location in membranes needs to be addressed. We have used a combination of dual polarisation interferometry (DPI), surface plasmon resonance spectroscopy (SPR) and atomic force microscopy (AFM) to gain an unprecedented detailed molecular picture of membrane lysis by antimicrobial peptides.

Results and Discussion

Dual Polarisation Interferometry

DPI is a new optical biosensor that provides a measure of the thickness and density of the membrane upon peptide binding, which together with the kinetic data from SPR and the high resolution images from AFM, provides an integrated approach to delineating specific molecular...

Keywords

Atomic Force Microscopy Surface Plasmon Resonance Antimicrobial Peptide Interference Pattern Slab Waveguide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The support of the Australian Research Council is gratefully acknowledged.

References

  1. 1.
    Popplewell, M.J. et al Biochim Biophys Acta. 1768, 13–20 (2007).CrossRefGoogle Scholar
  2. 2.
    Mozsolits, H. et al., Biochim Biophys Acta, 1512, 64–76 (2001).CrossRefGoogle Scholar
  3. 3.
    Mozsolits, H. and Aguilar, M. I., Biopolymers — Peptide Science, 66, 3–18 (2002).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Tzong-Hsien Lee
    • 1
  • Kristopher Hall
    • 1
  • Adam Mechler
    • 2
  • Lisandra Martin
    • 2
  • Jonathan Popplewell
    • 3
  • Gerry Ronan
    • 3
  • Marie-Isabel Aguilar
    • 1
  1. 1.Department of Biochemistry & Molecular BiologyMonash UniversityClayton VicAustralia
  2. 2.School of ChemistryMonash UniversityClayton VicAustralia
  3. 3.Farfield Scientific LtdCrewe

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