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Peptide-Chitosan Matrix: a New Multifunctional Biomaterial

  • Motoyoshi Nomizu
  • Natsumi Yamagata
  • Mayumi Mochizuki
  • Yamato Kikkawa
  • Yuichi Kadoya
Part of the Advances in Experimental Medicine and Biology book series (volume 611)

Introduction

Tissue engineering requires biomaterials for delivering transplanted cells to organ sites needing repair/regeneration. Extracellular matrix (ECM) plays a critical role in tissue regeneration by promoting cell adhesion, migration, proliferation, and differentiation. ECM mimetics are of importance for tissue engineering because of their functions as scaffolds for cells. Previously, we developed bioactive laminin-derived peptide-conjugated chitosan membranes and demonstrated their cell- and peptide-type specific functions [1]. We also demonstrated that a most active laminin peptide (AG73: RKRLQVQLSIRT)-conjugated chitosan membrane can deliver cells and is applicable for keratinocyte transferring to wound bed [2]. Here, we conjugated four integrin-binding peptides derived from ECM proteins onto chitosan membranes and examined biological activity. FIB1 (YAVTGRGDSPAS; from fibronectin), EF1zz (ATLQLQEGRLHFXFDLGKGR; from laminin α1 chain), A99 (AGTFALRGDNPQG; from laminin α1...

Keywords

PC12 Cell Neurite Outgrowth Human Foreskin Fibroblast Integrin Binding Chitosan Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Motoyoshi Nomizu
    • 1
  • Natsumi Yamagata
    • 1
  • Mayumi Mochizuki
    • 1
  • Yamato Kikkawa
    • 1
  • Yuichi Kadoya
    • 2
  1. 1.Toyko University of Pharmacy and Life scienceHachiojiJapan
  2. 2.Kitasato University School of MedicineSagamiharaJapan

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