Peptide-Chitosan Matrix: a New Multifunctional Biomaterial
Tissue engineering requires biomaterials for delivering transplanted cells to organ sites needing repair/regeneration. Extracellular matrix (ECM) plays a critical role in tissue regeneration by promoting cell adhesion, migration, proliferation, and differentiation. ECM mimetics are of importance for tissue engineering because of their functions as scaffolds for cells. Previously, we developed bioactive laminin-derived peptide-conjugated chitosan membranes and demonstrated their cell- and peptide-type specific functions . We also demonstrated that a most active laminin peptide (AG73: RKRLQVQLSIRT)-conjugated chitosan membrane can deliver cells and is applicable for keratinocyte transferring to wound bed . Here, we conjugated four integrin-binding peptides derived from ECM proteins onto chitosan membranes and examined biological activity. FIB1 (YAVTGRGDSPAS; from fibronectin), EF1zz (ATLQLQEGRLHFXFDLGKGR; from laminin α1 chain), A99 (AGTFALRGDNPQG; from laminin α1...
KeywordsPC12 Cell Neurite Outgrowth Human Foreskin Fibroblast Integrin Binding Chitosan Membrane
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