Stimuli-responsive Peptide Self-assembly into Well-organized Nanofibers

  • Tomoyuki Koga
  • Ken-ichi Kitamura
  • Nobuyuki Higashi
Part of the Advances in Experimental Medicine and Biology book series (volume 611)


Advances in peptide self-assembly provide strong incentives to fabricate novel nanostructured biomaterials. Recently, we have reported that fine tuning of peptide sequence, stereochemistry, and solution pH enables the construction of 3D-nanoarchitectures, including supramolecular nanofibers, by controlled self-assembly of 16-mer peptide building blocks [ 1, 2]. Here, we report a novel stimuli-responsive self-assembling system by using artificial peptides having enzyme-active site in order to control spontaneous assembling process. Amphiphilic block peptides 1 and 2, in which triblock-type β-sheet forming peptide (L 4K 8L 4) was conjugated with relatively hydrophilic poly(ethylene glycol) ( PEG) unit [3] or D,L-alternating oligolysine unit through thrombin-cleavable site (VPRGS), were designed and employed as building blocks for self-assembly (Fig. 1). The conformational and self-assembling properties of 1 and 2in response to enzyme triggering were investigated, especially in...


Random Coil Structure Negative Maximum Prepare Sample Solution Standard Solid Phase Hydrophilic Unit 
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This work was supported in part by a grant-in-aid for scientific research (No. 19710099), and by the Academic Frontier Research Project on “New Frontier of Biomedical Engineering Research” from the Ministry of Education, Culture, Sports, Science and Technology, Japan.


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    Koga, T., Kitamura, K. and Higashi, N. Chem. Commun. 4897–4899 (2006).Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Tomoyuki Koga
    • 1
  • Ken-ichi Kitamura
    • 1
  • Nobuyuki Higashi
    • 1
  1. 1.Department of Molecular Science & TechnologyFaculty of Engineering, Doshisha UniversityKyotanabeJAPAN

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