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Biophysical Aspects of Actin-Based Cell Motility in Fish Epithelial Keratocytes

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Cell Motility

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

Summary

Cell motility is a fascinating dynamic process crucial for a wide variety of biological phenomena, including defense against injury or infection, embryogenesis, and cancer metastasis. A cell using actin-based motility to crawl across a substrate must coordinate the action of numerous individual molecular building blocks to achieve coherent cell movement. While the molecular basis of cell motility is beginning to be understood, relatively little is known about the large-scale mechanisms responsible for this remarkable self-organization that bridges many orders of magnitude in both space and time. In this chapter, we discuss the biophysical aspects of actin-based cell motility and the importance of their interplay with the underlying biochemical processes, focusing on fish epithelial keratocytes as a relatively simple model system. We review the current understanding regarding the mechanical and biochemical aspects of keratocyte motility, and at the same time highlight some gaps in our knowledge.

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References

  1. Lauffenburger, D & Horwitz, A. (1996) Cell 84, 359–69.

    Article  Google Scholar 

  2. Alberts, B & et al. (2002) Molecular Biology of the Cell, 4th ed. (Garland, New York).

    Google Scholar 

  3. Geiger, B, Bershadsky, A, Pankov, R, & Yamada, K. (2001) Nat. Rev. Mol. Cell. Biol. 2, 793–805.

    Article  Google Scholar 

  4. Bershadsky, A, Balaban, N, & Geiger, B. (2003) Annu. Rev. Cell. Dev. Biol. 19, 677–95.

    Article  Google Scholar 

  5. Pantaloni, D, Le Clainche, C, & Carlier, M. (2001) Science 292, 1502–6.

    Article  ADS  Google Scholar 

  6. Pollard, T & Borisy, G. (2003) Cell 112, 453–65.

    Article  Google Scholar 

  7. Rafelski, S & Theriot, J. (2004) Ann. Rev. Biochem. 73.

    Google Scholar 

  8. Ridley, A, Schwartz, M, Burridge, K, Firtel, R, Ginsberg, M, Borisy, G, Parsons, J, & Horwitz, A. (2003) Science 302, 1704–9.

    Article  ADS  Google Scholar 

  9. Loisel, T, Boujemaa, R, Pantaloni, D, & Carlier, M. (1999) Nature 401, 613–6.

    Article  ADS  Google Scholar 

  10. van Oudenaarden, A & Theriot, J. (1999) Nat. Cell. Biol. 1, 493–9.

    Article  Google Scholar 

  11. Gerbal, F, Chaikin, P, Rabin, Y, & Prost, J. (2000) Biophys. J. 79, 2259–75.

    Article  Google Scholar 

  12. Bernheim-Groswasser, A, Wiesner, S, Golsteyn, R, Carlier, M, & Sykes, C. (2002) Nature 417, 308–11.

    Article  ADS  Google Scholar 

  13. Giardini, P, Fletcher, D, & Theriot, J. (2003) Proc. Natl. Acad. Sci. USA 100, 6493–8.

    Article  ADS  Google Scholar 

  14. Upadhyaya, A, Chabot, J, Andreeva, A, Samadani, A, & van Oudenaarden, A. (2003) Proc. Natl. Acad. Sci. USA 100, 4521–6.

    Article  ADS  Google Scholar 

  15. Wiesner, S, Helfer, E, Didry, D, Ducouret, G, Lafuma, F, Carlier, M, & Pantaloni, D. (2003) J. Cell. Biol. 160, 387–98.

    Article  Google Scholar 

  16. Marcy, Y, Prost, J, Carlier, M.-F, & Sykes, C. (2004) Proc. Natl. Acad. Sci. USA 101, 5992–5997.

    Article  ADS  Google Scholar 

  17. Alberts, J & Odell, G. (2004) PloS Biol. 2, e412.

    Article  Google Scholar 

  18. Goodrich, H. (1924) Biolog. Bull. 46, 252–262.

    Article  Google Scholar 

  19. Lee, J & Jacobson, K. (1997) J. Cell. Sci. 110, 2833–44.

    Google Scholar 

  20. Svitkina, T & Borisy, G. (1999) J. Cell. Biol. 145, 1009–26.

    Article  Google Scholar 

  21. Mogilner, A & Edelstein-Keshet, L. (2002) Biophys. J. 83, 1237–58.

    Article  Google Scholar 

  22. Grimm, H, Verkhovsky, A, Mogilner, A, & Meister, J. (2003) Europ. Biophys. J. 32, 563–77.

    Article  Google Scholar 

  23. Rubinstein, B, Jacobson, K, & Mogilner, A. (2005) Multisc. Model. Simul. 3, 413–439.

    Article  MATH  MathSciNet  Google Scholar 

  24. Lacayo, C. I, Pincus, Z, van Duijn, M, Wilson, C, Fletcher, D, Gertler, F, Mogilner, A, & Theriot, J. (2007) PLoS biology, in press.

    Google Scholar 

  25. Kunzenbacher, I, Bereiter-Hahn, J, Osborn, M, & Weber, K. (1982) Cell Tiss. Res. 222, 445–57.

    Article  Google Scholar 

  26. Strohmeier, R & Bereiter-Hahn, J. (1991) Cell Tissue Res. 266, 615–21.

    Article  Google Scholar 

  27. Radice, G. (1980) J. Cell. Sci. 44, 201–23.

    Google Scholar 

  28. Radice, G. (1980) Develop. Biol. 76, 26–46.

    Article  Google Scholar 

  29. Euteneuer, U & Schliwa, M. (1984) Nature 310, 58–61.

    Article  ADS  Google Scholar 

  30. Verkhovsky, A, Svitkina, T, & Borisy, G. (1999) Curr. Biol. 9, 11–20.

    Article  Google Scholar 

  31. Yam, P, Wilson, C. A, Ji, L, Hebert, B, Barnhart, E, Wiseman, P, Danuser, G, & Theriot, J. (2007) Submitted.

    Google Scholar 

  32. Cooper, M & Schliwa, M. (1985) J.Neurosci. Res. 13, 223–44.

    Article  Google Scholar 

  33. Cooper, M & Schliwa, M. (1986) J. Cell. Biol. 102, 1384–99.

    Article  Google Scholar 

  34. Theriot, J & Mitchison, T. (1991) Nature 352, 126–31.

    Article  ADS  Google Scholar 

  35. Small, J, Herzog, M, & Anderson, K. (1995) J. Cell. Biol. 129, 1275–86.

    Article  Google Scholar 

  36. Lee, J, Leonard, M, Oliver, T, Ishihara, A, & Jacobson, K. (1994) J. Cell. Biol. 127, 1957–64.

    Article  Google Scholar 

  37. Anderson, K, Wang, Y, & Small, J. (1996) J. Cell Biol. 134, 1209–18.

    Article  Google Scholar 

  38. Svitkina, T, Verkhovsky, A, McQuade, K, & Borisy, G. (1997) J. Cell. Biol. 139, 397–415.

    Article  Google Scholar 

  39. Lee, J, Ishihara, A, Theriot, J, & Jacobson, K. (1993) Nature 362, 167–71.

    Article  ADS  Google Scholar 

  40. Burton, K, Park, J, & Taylor, D. (1999) Mol. Bio. Cell. 10, 3745–69.

    Google Scholar 

  41. Oliver, T, Dembo, M, & K., J. (1999) J. Cell. Biol. 145, 589–604.

    Google Scholar 

  42. Abraham, V, Krishnamurthi, V, Taylor, D, & Lanni, F. (1999) Biophys. J. 77, 1721–32.

    Article  Google Scholar 

  43. Ream, R, Theriot, J, & Somero, G. (2003) J. Exp. Biol. 206, 4539–51.

    Article  Google Scholar 

  44. Pincus, Z & Theriot, J. (2007) J. Microsc. 227, 140–156.

    Article  MathSciNet  Google Scholar 

  45. Pollard, T, Blanchoin, L, & Mullins, R. (2000) Annu. Rev. Biophys. Biomol. Struct. A 29, 545–76.

    Article  Google Scholar 

  46. Huxley, H. (1963) J. Mol. Biol. 7, 281–308.

    Article  Google Scholar 

  47. Pollard, T & Mooseker, M. (1981) J. Cell Biol. 88, 654–659.

    Article  Google Scholar 

  48. Theriot, J. (2000) Traffic 1, 19–28.

    Article  Google Scholar 

  49. Hill, T & Kirschner, M. (1982) Internat. Rev. Cyt. 78, 1–125.

    Article  Google Scholar 

  50. Cortese, J, Schwab, B, Frieden, C, & Elson, E. (1989) Proc. Natl. Acad. Sci. USA 86, 5773–7.

    Article  ADS  Google Scholar 

  51. Miyata, H, Nishiyama, S, Akashi, K, & Kinosita, K. J. (1999) Proc. Natl. Acad. Sci. USA 96, 2048–53.

    Article  ADS  Google Scholar 

  52. Limozin, L & Sackmann, E. (2002) Phys. Rev. Lett. 89, 168103.

    Article  ADS  Google Scholar 

  53. Mogilner, A & Oster, G. (1996) Biophys. J. 71, 3030–3045.

    Article  ADS  Google Scholar 

  54. Mullins, R, Heuser, J, & Pollard, T. (1998) Proc. Natl. Acad. Sci. USA 95, 6181–6.

    Article  ADS  Google Scholar 

  55. Maly, I & Borisy, G. (2001) Proc. Natl. Acad. Sci. USA 98, 11324–9.

    Article  ADS  Google Scholar 

  56. Salmon, W, Adams, M, & Waterman-Storer, C. (2002) J. Cell. Biol. 158, 31–7.

    Article  Google Scholar 

  57. Watanabe, N & Mitchison, T. (2002) Science 295, 1083–6.

    Article  ADS  Google Scholar 

  58. Waterman-Storer, C, Desai, A, Bulinski, J, & E.D., S. (1998) Curr. Biol. 8, 1227–3026.

    Google Scholar 

  59. Ji, L & Danuser, G. (2005) J. Microsc. 220, 150–67.

    Article  MathSciNet  Google Scholar 

  60. Danuser, G & Waterman-Storer, C. (2006) Annu. Rev. Biophys. Biomol. Struct. 35, 361–87.

    Article  Google Scholar 

  61. Wilson, C & Theriot, J. (2006) IEEE Trans. Image. Process 15, 1939–51.

    Article  ADS  Google Scholar 

  62. Jurado, C, Haserick, J, & Lee, J. (2005) Mol. Biol. Cell. 16, 507–18.

    Article  Google Scholar 

  63. Vallotton, P, Danuser, G, Bohnet, S, Meister, J.-J, & Verkhovsky, A. (2005) Mol. Biol. Cell 16, 1223–1231.

    Article  Google Scholar 

  64. Tilney, L, Bonder, E, Coluccio, L, & Mooseker, M. (1983) J. Cell. Biol. 97, 112–24.

    Article  Google Scholar 

  65. Pollard, T & Cooper, J. (1984) Biochem. 23, 6631–6641.

    Article  Google Scholar 

  66. Carlier, M, Laurent, V, Santolini, J, Melki, R, Didry, D, Xia, G, Hong, Y, Chua, N, & Pantaloni, D. (1997) J. Cell. Biol. 136, 1307–22.

    Article  Google Scholar 

  67. Rogers, S, Wiedemann, U, Stuurman, N, & Vale, R. (2003) J. Cell. Biol. 162, 1079–88.

    Article  Google Scholar 

  68. Bear, J, Loureiro, J, Libova, I, Fassler, R, Wehland, J, & Gertler, F. (2000) Cell 101, 717–28.

    Article  Google Scholar 

  69. Bear, J, Svitkina, T, Krause, M, Schafer, D, Loureiro, J, Strasser, G, Maly, I, Chaga, O, Cooper, J, Borisy, G, & Gertler, F. (2002) Cell 109, 509–21.

    Article  Google Scholar 

  70. Han, Y, Chung, C, Wessels, D, Stephens, S, Titus, M, Soll, D, & Firtel, R. (2002) J. Biol. Chem. 277, 49877–87.

    Article  Google Scholar 

  71. Prass, M, Jacobson, K, Mogilner, A, & Radmacher, M. (2006) J. Cell. Biol. 174, 767–72.

    Article  Google Scholar 

  72. Parekh, S, Chaudhuri, O, Theriot, J, & Fletcher, D. (2005) Nat. Cell. Biol. 7, 1219–23.

    Article  Google Scholar 

  73. Sastry, S & Burridge, K. (2000) Exp. Cell Res. 261, 25–36.

    Article  Google Scholar 

  74. Izzard, C & Lochner, L. (1976) J. Cell. Sci. 21, 129–59.

    Google Scholar 

  75. Anderson, K & Cross, R. (2000) Curr. Biol. 10, 253–60.

    Article  Google Scholar 

  76. Bohnet, S, Ananthakrishnan, R, Mogilner, A, Meister, J, & Verkhovsky, A. (2006) Biophys. J. 90, 1810–20.

    Article  ADS  Google Scholar 

  77. Balaban, N, Schwarz, U, Riveline, D, Goichberg, P, Tzur, G, Sabanay, I, 0Mahalu, D, Safran, S, Bershadsky, A, Addadi, L, & Geiger, B. (2001) Nat. Cell Biol. 3, 466–72.

    Article  Google Scholar 

  78. Riveline, D, Zamir, E, Balaban, N, Schwarz, U, Ishizaki, T, Narumiya, S, Kam, Z, Geiger, B, & Bershadsky, A. (2001) J. Cell. Biol. 153, 1175–86.

    Article  Google Scholar 

  79. Doyle, A & Lee, J. (2002) Biotechn. 33, 358–64.

    Google Scholar 

  80. Doyle, A & Lee, J. (2005) J. Cell. Sci. 118, 369–379.

    Article  Google Scholar 

  81. Straight, A, Cheung, A, Limouze, J, Chen, I, Westwood, N, Sellers, J, & Mitchison, T. (2003) Science 299, 1743–7.

    Article  ADS  Google Scholar 

  82. De Lozanne, A & Spudich, J. (1987) Science 236, 1086–91.

    Article  ADS  Google Scholar 

  83. Knecht, D & Loomis, W. (1987) Science 236, 1081–6.

    Article  ADS  Google Scholar 

  84. Lee, J, Ishihara, A, Oxford, G, Johnson, B, & Jacobson, K. (1999) Nature 400, 382–6.

    Article  ADS  Google Scholar 

  85. Wolgemuth, C, Miao, L, Vanderlinde, O, Roberts, T, & Oster, G. (2005) Biophys. J. 88, 2462–2471.

    Article  Google Scholar 

  86. Bottino, D, Mogilner, A, Roberts, T, Stewart, M, & Oster, G. (2002) J. Cell. Sci. 115, 367–384.

    Google Scholar 

  87. Miao, L, Vanderlinde, O, Stewart, M, & Roberts, T. (2003) Science 302, 1405–7.

    Article  ADS  Google Scholar 

  88. Raucher, D & Sheetz, M. (2000) J. Cell. Biol. 148, 127–36.

    Article  Google Scholar 

  89. Ghosh, P, Vasanji, A, Murugesan, G, Eppell, S, Graham, L, & Fox, P. (2002) Nat. Cell. Bio. 4, 894–900.

    Article  Google Scholar 

  90. Schwab, A, Nechyporuk-Zloy, V, Fabian, A, & Stock, C. (2006) Pflugers Arch.

    Google Scholar 

  91. Bereiter-Hahn, J, Strohmeier, R, Kunzenbacher, I, Beck, K, & Voth, M. (1981) J. Cell Sci. 52, 289–311.

    Google Scholar 

  92. Odell, E & Oster, G. (1994) in Some Mathematical Problems in Biology, vol. 24., eds. Goldstein, B & Wofy, C. (American Mathematical Society., Providence, RI.), pp. 23–36.

    Google Scholar 

  93. Gov, N & Gopinathan, A. (2006) Biophys. J. 90, 454–69.

    Article  ADS  Google Scholar 

  94. Weisswange, I, Bretschneider, T, & Anderson, K. (2005) J. Cell. Sci. 118, 4375–80.

    Article  Google Scholar 

  95. Saadoun, S, Papadopoulos, M, Hara-Chikuma, M, & Verkman, A. (2005) Nature 434, 786–92.

    Article  ADS  Google Scholar 

  96. Kucik, D, Elson, E, & Sheetz, M. (1989) Nature 340, 315–7.

    Article  ADS  Google Scholar 

  97. Kucik, D, Elson, E, & Sheetz, M. (1990) J. Cell. Biol. 111, 1617–22.

    Article  Google Scholar 

  98. Popov, S, Brown, A, & Poo, M. (1993) Science 259, 244–6.

    Article  ADS  Google Scholar 

  99. Dai, J & Sheetz, M. (1995) Cell 83, 693–701.

    Article  Google Scholar 

  100. Charras, G, Yarrow, J, Horton, M, Mahadevan, L, & Mitchison, T. (2005) Nature 435, 365–9.

    Article  ADS  Google Scholar 

  101. Oster, G & Perelson, A. (1987) J. Cell. Sci. Suppl. 8, 35–54.

    Google Scholar 

  102. Maurel, C. (1997) Annu. Rev. Plant. Physiol. Plant. Mol. Biol. 48, 399–429.

    Article  Google Scholar 

  103. Loitto, V, Forslund, T, Sundqvist, T, Magnusson, K, & Gustafsson, M. (2002) J. Leuk. Biol. 71, 212–22.

    Google Scholar 

  104. Zicha, D, Dobbie, I, Holt, M, Monypenny, J, Soong, D, Gray, C, & Dunn, G. (2003) Science 300, 142–5.

    Article  ADS  Google Scholar 

  105. Schwartz, M & Horwitz, A. (2006) Cell 125, 1223–5.

    Article  Google Scholar 

  106. Gupton, S & Waterman-Storer, C. (2006) Cell 125, 1361–74.

    Article  Google Scholar 

  107. Wang, F, Herzmark, P, Weiner, O, Srinivasan, S, Servant, G, & Bourne, H. (2002) Nat. Cell. Biol. 4, 513–8.

    Google Scholar 

  108. Janmey, P, Hvidt, S, Kas, J, Lerche, D, Maggs, A, Sackmann, E, Schliwa, M, & Stossel, T. (1994) J. Biol. Chem. 269, 32503–13.

    Google Scholar 

  109. Carlsson, A. (2001) Biophys. J. 81, 1907–23.

    Article  ADS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Stanford University, Stanford, CA 94305, USA

    Kinneret Keren & Julie A. Theriot

  2. Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA

    Julie A. Theriot

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  1. Kinneret Keren
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  2. Julie A. Theriot
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Keren, K., Theriot, J.A. (2008). Biophysical Aspects of Actin-Based Cell Motility in Fish Epithelial Keratocytes. In: Cell Motility. Biological and Medical Physics, Biomedical Engineering. Springer, New York, NY. https://doi.org/10.1007/978-0-387-73050-9_2

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