OGG1: From Structural Analysis to the Knockout Mouse

  • Arne Klungland
  • Jon K. Laerdahl
  • Torbjørn Rognes
Part of the Molecular Biology Intelligence Unit book series (MBIU)


Among the four DNA bases, guanine, having the lowest redox potential, is the most susceptible to oxidation, and among the oxidized bases 7,8-dihydro-8-oxoguanine (8-oxoG) is certainly the lesion that has retained most attention over many years. This altered base can pair with A as well as C residues during replication. Eukaryotic cells use a specific DNA glycosylase, 8-oxoG DNA glycosylase (OGG1), to excise 8-oxoG from DNA, and repair-deficient cells are characterized by an increased G to T transversion frequency. It is essential that OGG1 has the ability to distinguish between 8-oxoG:C and 8-oxoG:A pairs and only removes 8-oxoG paired with C. This review will discuss the structural basis for OGG1 damage recognition and specificity as well as the literature on the biological consequences of OGG1 deficiency.


Base Excision Repair Cockayne Syndrome Glycosylase Activity OGG1 Ser326Cys Spontaneous Mutation Frequency 


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  1. 1.
    Cadet J, Berger M, Douki T et al. Oxidative damage to DNA: Formation, measurement, and biological significance. Rev Physiol Biochem Pharmacol 1997; 131:1–87.PubMedGoogle Scholar
  2. 2.
    Shibutani S, Takeshita M, Grollman AP. Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG. Nature 1991; 349:431–434.PubMedCrossRefGoogle Scholar
  3. 3.
    Boiteux S, O’Connor TR, Laval J. Formamidopyrimidine-DNA glycosylase of Escherichia coli: Cloning and sequencing of the fpg structural gene and overproduction of the protein. EMBO J 1987; 6:3177–3183.PubMedGoogle Scholar
  4. 4.
    Michaels ML, Pham L, Nghiem Y et al. MutY, an adenine glycosylase active on G-A mispairs, has homology to endonuclease III. Nucleic Acids Res 1990; 18:3841–3845.PubMedCrossRefGoogle Scholar
  5. 5.
    Michaels ML, Cruz C, Grollman AP et al. Evidence that MutY, and MutM combine to prevent mutations by an oxidatively damaged form of guanine in DNA. Proc Natl Acad Sci USA 1992; 89:7022–7025.PubMedCrossRefGoogle Scholar
  6. 6.
    Stivers JT, Jiang YL. A mechanistic perspective on the chemistry of DNA repair glycosylases. Chem Rev 2003; 103:2729–2759.PubMedCrossRefGoogle Scholar
  7. 7.
    Fortini P, Pascucci B, Parlanti E et al. 8-Oxoguanine DNA damage: At the crossroad of alternative repair pathways. Mutat Res 2003; 531:127–139.PubMedGoogle Scholar
  8. 8.
    Pascucci B, Maga G, Hubscher U et al. Reconstitution of the base excision repair pathway for 7,8-dihydro-8-oxoguanine with purified human proteins. Nucleic Acids Res 2002; 30:2124–2130.PubMedCrossRefGoogle Scholar
  9. 9.
    Seeberg E, Luna L, Morland I et al. Base removers and strand scissors: Different strategies employed in base excision and strand incision at modified base residues in DNA. Cold Spring Harb Symp Quant Biol 2000; 65:135–142.PubMedCrossRefGoogle Scholar
  10. 10.
    McCullough AK, Sanchez A, Dodson ML et al. The reaction mechanism of DNA glycosylase/AP lyases at abasic sites. Biochemistry 2001; 40:561–568.PubMedCrossRefGoogle Scholar
  11. 11.
    Dodson ML, Michaels ML, Lloyd RS. Unified catalytic mechanism for DNA glycosylases. J Biol Chem 1994; 269:32709–32712.PubMedGoogle Scholar
  12. 12.
    Boiteux S, Radicella JP. The human OGG1 gene: Structure, functions, and its implication in the process of carcinogenesis. Arch Biochem Biophys 2000; 377:1–8.PubMedCrossRefGoogle Scholar
  13. 13.
    Bjelland S, Seeberg E. Mutagenicity, toxicity and repair of DNA base damage induced by oxidation. Mutat Res 2003; 531:37–80.PubMedGoogle Scholar
  14. 14.
    Barnes DE, Lindahl T. Repair and genetic consequences of endogenous DNA base damage in mammalian cells. Annu Rev Genet 2004; 38:445–476.PubMedCrossRefGoogle Scholar
  15. 15.
    Evans MD, Dizdaroglu M, Cooke MS. Oxidative DNA damage and disease: Induction, repair and significance. Mutat Res 2004; 567:1–61.PubMedCrossRefGoogle Scholar
  16. 16.
    Slupphaug G, Kavli B, Krokan HE. The interacting pathways for prevention and repair of oxidative DNA damage. Mutat Res 2003; 531:231–251.PubMedGoogle Scholar
  17. 17.
    van der Kemp PA, Thomas D, Barbey R et al. Cloning and expression in Escherichia coli of the OGG1 gene of Saccharomyces cerevisiae, which codes for a DNA glycosylase that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine. Proc Natl Acad Sci USA 1996; 93:5197–5202.PubMedCrossRefGoogle Scholar
  18. 18.
    Berdal KG, Bjoras M, Bjelland S et al. Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene. EMBO J 1990; 9:4563–4568.PubMedGoogle Scholar
  19. 19.
    Chen J, Derfler B, Samson L. Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology to the AlkA glycosylase of E. coli and is induced in response to DNA alkylation damage. EMBO J 1990; 9:4569–4575.PubMedGoogle Scholar
  20. 20.
    Chen J, Derfler B, Maskati A et al. Cloning a eukaryotic DNA glycosylase repair gene by the suppression of a DNA repair defect in Escherichia coli. Proc Natl Acad Sci USA 1989; 86:7961–7965.PubMedCrossRefGoogle Scholar
  21. 21.
    Nash HM, Bruner SD, Scharer OD et al. Cloning of a yeast 8-oxoguanine DNA glycosylase reveals the existence of a base-excision DNA-repair protein superfamily. Curr Biol 1996; 6:968–980PubMedCrossRefGoogle Scholar
  22. 22.
    Aburatani H, Hippo Y, Ishida T et al. Cloning and characterization of mammalian 8-hydroxyguanine-specific DNA glycosylase/apurinic, apyrimidinic lyase, a functional mutM homologue. Cancer Res 1997; 57:2151–2156.PubMedGoogle Scholar
  23. 23.
    Arai K, Morishita K, Shinmura K et al. Cloning of a human homolog of the yeast OGG1 gene that is involved in the repair of oxidative DNA damage. Oncogene 1997; 14:2857–2861.PubMedCrossRefGoogle Scholar
  24. 24.
    Bjoras M, Luna L, Johnsen B et al. Opposite base-dependent reactions of a human base excision repair enzyme on DNA containing 7,8-dihydro-8-oxoguanine and abasic sites. EMBO J 1997; 16:6314–6322.PubMedCrossRefGoogle Scholar
  25. 25.
    Lu R, Nash HM, Verdine GL. A mammalian DNA repair enzyme that excises oxidatively damaged guanines maps to a locus frequently lost in lung cancer. Curr Biol 1997; 7:397–407.PubMedCrossRefGoogle Scholar
  26. 26.
    Radicella JP, Dherin C, Desmaze C et al. Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae. Proc Natl Acad Sci USA 1997; 94:8010–8015.PubMedCrossRefGoogle Scholar
  27. 27.
    Roldan-Arjona T, Wei YF, Carter KC et al. Molecular cloning and functional expression of a human cDNA encoding the antimutator enzyme 8-hydroxyguanine-DNA glycosylase. Proc Natl Acad Sci USA 1997; 94:8016–8020.PubMedCrossRefGoogle Scholar
  28. 28.
    Rosenquist TA, Zharkov DO, Grollman AP. Cloning and characterization of a mammalian 8-oxoguanine DNA glycosylase. Proc Natl Acad Sci USA 1997; 94:7429–7434.PubMedCrossRefGoogle Scholar
  29. 29.
    Kuo FC, Sklar J. Augmented expression of a human gene for 8-oxoguanine DNA glycosylase (MutM) in B lymphocytes of the dark zone in lymph node germinal centers. J Exp Med 1997; 186:1547–1556.PubMedCrossRefGoogle Scholar
  30. 30.
    Nishioka K, Ohtsubo T, Oda H et al. Expression and differential intracellular localization of two major forms of human 8-oxoguanine DNA glycosylase encoded by alternatively spliced OGG1 mRNAs. Mol Biol Cell 1999; 10:1637–1652.PubMedGoogle Scholar
  31. 31.
    Girard PM, D’Ham C, Cadet J et al. Opposite base-dependent excision of 7,8-dihydro-8-oxoadenine by the Ogg1 protein of Saccharomyces cerevisiae. Carcinogenesis 1998; 19:1299–1305.PubMedCrossRefGoogle Scholar
  32. 32.
    Zharkov DO, Rosenquist TA, Gerchman SE et al. Substrate specificity and reaction mechanism of murine 8-oxoguanine-DNA glycosylase J Biol Chem 2000; 275:28607–28617.PubMedCrossRefGoogle Scholar
  33. 33.
    Jensen A, Calvayrac G, Karahalil B et al. Mammalian 8-oxoguanine DNA glycosylase 1 incises 8-oxoadenine opposite cytosine in nuclei and mitochondria, while a different glycosylase incises 8-oxoadenine opposite guanine in nuclei. J Biol Chem 2003; 278:19541–19548.PubMedCrossRefGoogle Scholar
  34. 34.
    Jaruga P, Dizdaroglu M. Repair of products of oxidative DNA base damage in human cells. Nucleic Acids Res 1996; 24:1389–1394.PubMedCrossRefGoogle Scholar
  35. 35.
    Kamiya H, Murata-Kamiya N, Koizume S et al. 8-Hydroxyguanine (7,8-dihydro-8-oxoguanine) in hot spots of the c-Ha-ras gene: Effects of sequence contexts on mutation spectra. Carcinogenesis 1995; 16:883–889.PubMedCrossRefGoogle Scholar
  36. 36.
    Kamiya H, Miura H, Murata-Kamiya N et al. 8-Hydroxyadenine (7,8-dihydro-8-oxoadenine) induces misincorporation in in vitro DNA synthesis and mutations in NIH 3T3 cells. Nucleic Acids Res 1995; 23:2893–2899.PubMedCrossRefGoogle Scholar
  37. 37.
    Bjoras M, Seeberg E, Luna L et al. Reciprocal “flipping” underlies substrate recognition and catalytic activation by the human 8-oxo-guanine DNA glycosylase. J Mol Biol 2002; 317:171–177.PubMedCrossRefGoogle Scholar
  38. 38.
    Banerjee A, Yang W, Karplus M et al. Structure of a repair enzyme interrogating undamaged DNA elucidates recognition of damaged DNA. Nature 2005; 434:612–618.PubMedCrossRefGoogle Scholar
  39. 39.
    Bruner SD, Norman DP, Verdine GL. Structural basis for recognition and repair of the endogenous mutagen 8-oxoguanine in DNA. Nature 2000; 403:859–866.PubMedCrossRefGoogle Scholar
  40. 40.
    Chung SJ, Verdine GL. Structures of end products resulting from lesion processing by a DNA glycosylase/lyase. Chem Biol 2004; 11:1643–1649.PubMedCrossRefGoogle Scholar
  41. 41.
    Fromme JC, Bruner SD, Yang W et al. Product-assisted catalysis in base-excision DNA repair. Nat Struct Biol 2003; 10:204–211.PubMedCrossRefGoogle Scholar
  42. 42.
    Norman DP, Chung SJ, Verdine GL. Structural and biochemical exploration of a critical amino acid in human 8-oxoguanine glycosylase. Biochemistry 2003; 42:1564–1572.PubMedCrossRefGoogle Scholar
  43. 43.
    Norman DP, Bruner SD, Verdine GL. Coupling of substrate recognition and catalysis by a human base-excision DNA repair protein. J Am Chem Soc 2001; 123:359–360.PubMedCrossRefGoogle Scholar
  44. 44.
    Chen L, Haushalter KA, Lieber CM et al. Direct visualization of a DNA glycosylase searching for damage. Chem Biol 2002; 9:345–350.PubMedCrossRefGoogle Scholar
  45. 45.
    Sartori AA, Lingaraju GM, Hunziker P et al. Pa-AGOG, the founding member of a new family of archaeal 8-oxoguanine DNA-glycosylases. Nucleic Acids Res 2004; 32:6531–6539.PubMedCrossRefGoogle Scholar
  46. 46.
    Doherty AJ, Serpell LC, Ponting CP. The helix-hairpin-helix DNA-binding motif: A structural basis for nonsequence-specific recognition of DNA. Nucleic Acids Res 1996; 24:2488–2497.PubMedCrossRefGoogle Scholar
  47. 47.
    Thayer MM, Ahern H, Xing D et al. Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structure. EMBO J 1995; 14:4108–4120.PubMedGoogle Scholar
  48. 48.
    Shinmura K, Kohno T, Kasai H et al. Infrequent mutations of the hOGG1 gene, that is involved in the excision of 8-hydroxyguanine in damaged DNA, in human gastric cancer. Jpn J Cancer Res 1998; 89:825–828.PubMedGoogle Scholar
  49. 49.
    Vidal AE, Hickson ID, Boiteux S et al. Mechanism of stimulation of the DNA glycosylase activity of hOGG1 by the major human AP endonuclease: Bypass of the AP lyase activity step. Nucleic Acids Res 2001; 29:1285–1292.PubMedCrossRefGoogle Scholar
  50. 50.
    Hill JW, Hazra TK, Izumi T et al. Stimulation of human 8-oxoguanine-DNA glycosylase by AP-endonuclease: Potential coordination of the initial steps in base excision repair. Nucleic Acids Res 2001; 29:430–438.PubMedCrossRefGoogle Scholar
  51. 51.
    Morland I, Luna I, Gustad E et al. Product inhibition and magnesium modulate the dual reaction mode of hOggl. DNA Repair (Amst) 2005; 4:381–387.CrossRefGoogle Scholar
  52. 52.
    Saitoh T, Shinmura K, Yamaguchi S et al. Enhancement of OGG1 protein AP lyase activity by increase of APEX protein. Mutat Res 2001; 486:31–40.PubMedGoogle Scholar
  53. 53.
    Marsin S, Vidal AE, Sossou M et al. Role of XRCC1 in the coordination and stimulation of oxidative DNA damage repair initiated by the DNA glycosylase hOGG1. J Biol Chem 2003; 278:44068–44074.PubMedCrossRefGoogle Scholar
  54. 54.
    Allinson SL, Dianova II, Dianov GL. DNA polymerase beta is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO J 2001; 20:6919–6926.PubMedCrossRefGoogle Scholar
  55. 55.
    Schyman P, Danielsson J, Pinak M et al. Theoretical study of the human DNA repair protein HOGG1 activity. J Phys Chem A 2005; 109:1713–1719.PubMedCrossRefGoogle Scholar
  56. 56.
    Dantzer F, Luna L, Bjoras M et al. Human OGG1 undergoes serine phosphorylation and associates with the nuclear matrix and mitotic chromatin in vivo. Nucleic Acids Res 2002; 30:2349–2357.PubMedCrossRefGoogle Scholar
  57. 57.
    Hu J, Imam SZ, Hashiguchi K et al. Phosphorylation of human oxoguanine DNA glycosylase (alpha-OGG1) modulates its function. Nucleic Acids Res 2005; 33:3271–3282.PubMedCrossRefGoogle Scholar
  58. 58.
    Luna L, Rolseth V, Hildrestrand GA et al. Dynamic relocalization of hOGG1 during the cell cycle is disrupted in cells harbouring the hOGG1-Cys326 polymorphic variant. Nucleic Acids Res 2005; 33:1813–1824.PubMedCrossRefGoogle Scholar
  59. 59.
    Nash HM, Lu R, Lane WS et al. The critical active-site amine of the human 8-oxoguanine DNA glycosylase, hOgg1: Direct identification, ablation and chemical reconstitution. Chem Biol 1997; 4:693–702.PubMedCrossRefGoogle Scholar
  60. 60.
    van der Kemp PA, Charbonnier JB, Audebert M et al. Catalytic and DNA-binding properties of the human Ogg1 DNA N-glycosylase/AP lyase: Biochemical exploration of H270, Q315 and F319, three amino acids of the 8-oxoguanine-binding pocket. Nucleic Acids Res 2004; 32:570–578.PubMedCrossRefGoogle Scholar
  61. 61.
    Hashiguchi K, Stuart JA, de Souza-Pinto NC et al. The C-terminal alphaO helix of human Ogg1 is essential for 8-oxoguanine DNA glycosylase activity: The mitochondrial beta-Ogg1 lacks this domain and does not have glycosylase activity. Nucleic Acids Res 2004; 32:5596–5608.PubMedCrossRefGoogle Scholar
  62. 62.
    Klungland A, Rosewell I, Hollenbach S et al. Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage. Proc Natl Acad Sci USA 1999; 96:13300–13305.PubMedCrossRefGoogle Scholar
  63. 63.
    Minowa O, Arai T, Hirano M et al. Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice. Proc Natl Acad Sci USA 2000; 97:4156–4161.PubMedCrossRefGoogle Scholar
  64. 64.
    Osterod M, Hollenbach S, Hengstler JG et al. Age-related and tissue-specific accumulation of oxidative DNA base damage in 7,8-dihydro-8-oxoguanine-DNA glycosylase (Ogg1) deficient mice. Carcinogenesis 2001; 22:1459–1463.PubMedCrossRefGoogle Scholar
  65. 65.
    Winter DB, Phung QH, Zeng X et al. Normal somatic hypermutation of Ig genes in the absence of 8-hydroxyguanine-DNA glycosylase. J Immunol 2003; 170:5558–5562.PubMedGoogle Scholar
  66. 66.
    Sakumi K, Tominaga Y, Furuichi M et al. Ogg1 knockout-associated lung tumorigenesis and its suppression by Mth1 gene disruption. Cancer Res 2003; 63:902–905.PubMedGoogle Scholar
  67. 67.
    Michaels ML, Miller JH. The GO system protects organisms from the mutagenic effect of the spontaneous lesion 8-hydroxyguanine (7,8-dihydro-8-oxoguanine). J Bacteriol 1992; 174:6321–6325.PubMedGoogle Scholar
  68. 68.
    Xie Y, Yang H, Cunanan C et al. Deficiencies in mouse Myh and Ogg1 result in tumor predisposition and G to T mutations in codon 12 of the K-ras oncogene in lung tumors. Cancer Res 2004; 64:3096–3102.PubMedCrossRefGoogle Scholar
  69. 69.
    Lipton L, Halford SE, Johnson V et al. Carcinogenesis in MYH-associated polyposis follows a distinct genetic pathway. Cancer Res 2003; 63:7595–7599.PubMedGoogle Scholar
  70. 70.
    Halford SE, Rowan AJ, Lipton L et al. Germline mutations but not somatic changes at the MYH locus contribute to the pathogenesis of unselected colorectal cancers. Am J Pathol 2003; 162:1545–1548.PubMedGoogle Scholar
  71. 71.
    de Souza-Pinto NC, Eide L, Hogue BA et al. Repair of 8-oxodeoxyguanosine lesions in mitochondrial DNA depends on the oxoguanine DNA glycosylase (OGG1) gene and 8-oxoguanine accumulates in the mitochondrial DNA of OGG1-defective mice. Cancer Res 2001; 61:5378–5381.PubMedGoogle Scholar
  72. 72.
    Le PF, Klungland A, Barnes DE et al. Transcription coupled repair of 8-oxoguanine in murine cells: The ogg1 protein is required for repair in nontranscribed sequences but not in transcribed sequences. Proc Natl Acad Sci USA 2000; 97:8397–8402.CrossRefGoogle Scholar
  73. 73.
    Osterod M, Larsen E, Le PF et al. A global DNA repair mechanism involving the Cockayne syndrome B (CSB) gene product can prevent the in vivo accumulation of endogenous oxidative DNA base damage. Oncogene 2002; 21:8232–8239.PubMedCrossRefGoogle Scholar
  74. 74.
    Tuo J, Chen C, Zeng X et al. Functional crosstalk between hOggl and the helicase domain of Cockayne syndrome group B protein. DNA Repair (Amst) 2002; 1:913–927.CrossRefGoogle Scholar
  75. 75.
    Larsen E, Kwon K, Coin F et al. Transcription activities at 8-oxoG lesions in DNA. DNA Repair (Amst) 2004; 3:1457–1468.CrossRefGoogle Scholar
  76. 76.
    Rozalski R, Siomek A, Gackowski D et al. Substantial decrease of urinary 8-oxo-7,8-dihydroguanine, a product of the base excision repair pathway, in DNA glycosylase defective mice. Int J Biochem Cell Biol 2005; 37:1331–1336.PubMedCrossRefGoogle Scholar
  77. 77.
    Nohmi T, Kim SR, Yamada M. Modulation of oxidative mutagenesis and carcinogenesis by polymorphic forms of human DNA repair enzymes. Mutat Res 2005; 591:60–73.PubMedGoogle Scholar
  78. 78.
    Xu J, Zheng SL, Turner A et al. Associations between hOGG1 sequence variants and prostate cancer susceptibility. Cancer Res 2002; 62:2253–2257.PubMedGoogle Scholar
  79. 79.
    Sugimura H, Kohno T, Wakai K et al. hOGG1 Ser326Cys polymorphism and lung cancer susceptibility. Cancer Epidemiol Biomarkers Prev 1999; 8:669–674.PubMedGoogle Scholar
  80. 80.
    Vogel U, Nexo BA, Olsen A et al. No association between OGG1 Ser326Cys polymorphism and breast cancer risk. Cancer Epidemiol Biomarkers Prev 2003; 12:170–171.PubMedGoogle Scholar
  81. 81.
    Vogel U, Olsen A, Wallin H et al. No association between OGG1 Ser326Cys and risk of basal cell carcinoma. Cancer Epidemiol Biomarkers Prev 2004; 13:1680–1681.PubMedGoogle Scholar
  82. 82.
    Vogel U, Nexo BA, Wallin H et al. No association between base excision repair gene polymorphisms and risk of lung cancer. Biochem Genet 2004; 42:453–460.PubMedCrossRefGoogle Scholar
  83. 83.
    Janssen K, Schlink K, Gotte W et al. DNA repair activity of 8-oxoguanine DNA glycosylase 1 (OGG1) in human lymphocytes is not dependent on genetic polymorphism Ser326/Cys326. Mutat Res 2001; 486:207–216.PubMedGoogle Scholar
  84. 84.
    Aspinwall R, Rothwell DG, Roldan-Arjona T et al. Cloning and characterization of a functional human homolog of Escherichia coli endonuclease III. Proc Natl Acad Sci USA 1997; 94:109–114.PubMedCrossRefGoogle Scholar
  85. 85.
    Eide L, Bjoras M, Pirovano M et al. Base excision of oxidative purine and pyrimidine DNA damage in Saccharomyces cerevisiae by a DNA glycosylase with sequence similarity to endonuclease III from Escherichia coli. Proc Natl Acad Sci USA 1996; 93:10735–10740.PubMedCrossRefGoogle Scholar
  86. 86.
    Luna L, Bjoras M, Hoff E et al. Cell-cycle regulation, intracellular sorting and induced overexpression of the human NTH1 DNA glycosylase involved in removal of formamidopyrimidine residues from DNA. Mutat Res 2000; 460:95–104.PubMedGoogle Scholar
  87. 87.
    Morland I, Rolseth V, Luna L et al. Human DNA glycosylases of the bacterial Fpg/MutM super-family: An alternative pathway for the repair of 8-oxoguanine and other oxidation products in DNA. Nucleic Acids Res 2002; 30:4926–4936.PubMedCrossRefGoogle Scholar
  88. 88.
    Bessho T, Roy R, Yamamoto K et al. Repair of 8-hydroxyguanine in DNA by mammalian N-methylpurine-DNA glycosylase. Proc Natl Acad Sci USA 1993; 90:8901–8904.PubMedCrossRefGoogle Scholar
  89. 89.
    Hang B, Singer B, Margison GP et al. Targeted deletion of alkylpurine-DNA-N-glycosylase in mice eliminates repair of 1,N6-ethenoadenine and hypoxanthine but not of 3,N4-ethenocytosine or 8-oxoguanine. Proc Natl Acad Sci USA 1997; 94:12869–12874.PubMedCrossRefGoogle Scholar
  90. 90.
    Hazra TK, Izumi T, Kow YW et al. The discovery of a new family of mammalian enzymes for repair of oxidatively damaged DNA, and its physiological implications. Carcinogenesis 2003; 24:155–157.PubMedCrossRefGoogle Scholar
  91. 91.
    Hazra TK, Izumi T, Boldogh I et al. Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA. Proc Natl Acad Sci USA 2002; 99:3523–3528.PubMedCrossRefGoogle Scholar
  92. 92.
    Dou H, Mitra S, Hazra TK. Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2. J Biol Chem 2003; 278:49679–49684.PubMedCrossRefGoogle Scholar
  93. 93.
    Boiteux S, Gellon L, Guibourt N. Repair of 8-oxoguanine in Saccharomyces cerevisiae: Interplay of DNA repair and replication mechanisms. Free Radic Biol Med 2002; 32:1244–1253.PubMedCrossRefGoogle Scholar
  94. 94.
    Vaisman A, Woodgate R. Unique misinsertion specificity of poliota may decrease the mutagenic potential of deaminated cytosines. EMBO J 2001; 20:6520–6529.PubMedCrossRefGoogle Scholar
  95. 95.
    Pruitt KD, Tatusova T, Maglott DR. NCBI Reference Sequence (RefSeq): A curated nonredundant sequence database of genomes, transcripts and proteins. Nucleic Acids Res 2005; 33:D501–D504.PubMedCrossRefGoogle Scholar
  96. 96.
    Hubbard T, Andrews D, Caccamo M et al. Ensembl 2005. Nucleic Acids Res 2005; 33:D447–D453.PubMedCrossRefGoogle Scholar
  97. 97.
    Edgar RC. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Ncleic Acids Res 2004; 32:1792–1797.CrossRefGoogle Scholar

Copyright information

© Landes Bioscience and Springer Science+Business Media 2007

Authors and Affiliations

  • Arne Klungland
    • 1
  • Jon K. Laerdahl
    • 1
  • Torbjørn Rognes
    • 1
  1. 1.Centre for Molecular Biology and Neuroscience and Institute of Medical MicrobiologyRikshospitalet-RadiumhospitaletOsloNorway

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