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Analysis of 8-Hydroxy-2′-Deoxyguanosine as a Marker of Oxidatively Damaged DNA in Relation to Carcinogenesis and Aging

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Oxidative Damage to Nucleic Acids

Part of the book series: Molecular Biology Intelligence Unit ((MBIU))

Abstract

Reactive oxygen species (ROS) are well known hazards for living organisms and are believed to be associated with the induction of cancer. ROS induce many forms of oxidative damage to proteins, nucleic acids, and lipids. Therefore, to diagnose or prevent cancer, analyses of oxidative products in patient samples, including tissue, blood, and urine, are very informative. Amongst the products of DNA oxidation, 8-hydroxy-2′-deoxyguanosine (8-OH-dG) is an important form of damage that leads to point mutations in genomic DNA. Since 8-OH-dG is the most abundant form of oxidative DNA damage and is easy to detect in laboratories, using a high performance liquid chromatography (HPLC) system equipped with an electrochemical detector (ECD), many researchers studying cellular oxidative stress have primarily analyzed 8-OH-dG. In this chapter, we will describe our findings regarding 8-OH-dG generation and its repair, as well as our recent urinary 8-OH-dG data.

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References

  1. Kasai H, Nishimura S. Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents. Nucleic Acids Res 1984; 12:2137–2145.

    Article  PubMed  CAS  Google Scholar 

  2. Cheng KC, Cahill DS, Kasai H et al. 8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G → T and A → C substitutions. J Biol Chem 1992; 267:166–172.

    PubMed  CAS  Google Scholar 

  3. Helbock HJ, Beckman KB, Shigenaga MK et al. DNA oxidation matters: The HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine. Proc Natl Acad Sci USA 1998; 95:288–293.

    Article  PubMed  CAS  Google Scholar 

  4. Nakae D, Mizumoto Y, Kobayashi E et al. Improved genomic/nuclear DNA extraction for 8-hydroxydeoxyguanosine analysis of small amounts of rat liver tissue. Cancer Lett 1995; 97:233–239.

    Article  PubMed  CAS  Google Scholar 

  5. Yamaguchi R, Hirano T, Asami S et al. Increased 8-hydroxyguanine levels in DNA and its repair activity in rat kidney after administration of a renal carcinogen, ferric nitrilotriacetate. Carcinogenesis 1996; 17:2419–2422.

    Article  PubMed  CAS  Google Scholar 

  6. Kasai H. A new automated method to analyze urinary 8-hydroxydeoxyguanosine by a high-performance liquid chromatography-electrochemical detector system. J Radiat Res (Tokyo) 2003; 44:185–189.

    Article  CAS  Google Scholar 

  7. Hirano T, Yamaguchi R, Asami S et al. 8-Hydroxyguanine levels in nuclear DNA and its repair activity in rat organs associated with age. J Gerontol 1996; 51A:B303–B307.

    CAS  Google Scholar 

  8. Yamaguchi R, Hirano T, Ootsuyama Y et al. Increased 8-hydroxyguanine in DNA and its repair activity in hamster and rat lung after intratracheal instillation of crocidolite asbestos. Jpn J Cancer Res 1999; 90:505–509.

    PubMed  CAS  Google Scholar 

  9. Hirano T, Yamaguchi Y, Kasai H. Inhibition of 8-hydroxyguanine repair in testes after administration of cadmium chloride to GSH-depleted rats. Toxicol Appl Pharmacol 1997; 147:9–14.

    Article  PubMed  CAS  Google Scholar 

  10. Tsurudome Y, Hirano T, Yamato H et al. Changes in levels of 8-hydroxyguanine in DNA, its repair and OGG1 mRNA in rat lungs after intratracheal administration of diesel exhaust particles. Carcinogenesis 1999; 20:1573–1576.

    Article  PubMed  CAS  Google Scholar 

  11. Hommma Y, Tsunoda M, Kasai H. Evidence for the accumulation of oxidative stress during cellular aging of human diploid fibroblasts. Biochem Biophys Res Commun 1994; 203:1063–1068.

    Article  Google Scholar 

  12. Mei N, Kunugita N, Hirano T et al. Acute arsenite-induced 8-hydroxyguanine is associated with inhibition of repair activity in cultured human cells. Biochem Biophys Res Commun 2002; 297:924–930.

    Article  PubMed  CAS  Google Scholar 

  13. Asami S, Hirano T, Yamaguchi R et al. Increase of a type of oxidative DNA damage, 8-hydroxyguanine, and its repair activity in human leukocytes by cigarette smoking. Cancer Res 1996; 56:2546–2549.

    PubMed  CAS  Google Scholar 

  14. Asami S, Manabe H, Miyabe J et al. Cigarette smoking induces an increase in oxidative DNA damage, 8-hydroxydeoxyguanosine, in a central site of the human lung. Carcinogenesis 1997; 18:1763–1766.

    Article  PubMed  CAS  Google Scholar 

  15. Inoue M, Osaki T, Noguchi M et al. Lung cancer patients have increased 8-hydroxydeoxyguanosine levels in peripheral lung tissue DNA. Jpn J Cancer Res 1998; 89:691–695.

    PubMed  CAS  Google Scholar 

  16. Tsurudome Y, Hirano T, Hirata K et al. Age-associated increase of 8-hydroxydeoxyguanosine in human colorectal tissue DNA. J Gerontol 2001; 56A:B483–B485.

    CAS  Google Scholar 

  17. Hirano T, Yamaguchi Y, Hirano H et al. Age-associated change of 8-hydroxyguanine repair activity in cultured human fibroblasts. Biochem Biophys Res Commun 1995; 214:1157–1162.

    Article  PubMed  CAS  Google Scholar 

  18. Lu R, Nash HM, Verdine GL. A mammalian DNA repair enzyme that excises oxidatively damaged guanines maps to a locus frequently lost in lung cancer. Curr Biol 1997; 7:397–407.

    Article  PubMed  CAS  Google Scholar 

  19. Rosenquist TA, Zharkov DO, Grollman AP. Cloning and characterization of a mammalian 8-oxoguanine DNA glycosylase. Proc Natl Acad Sci USA 1997; 94:7429–7434.

    Article  PubMed  CAS  Google Scholar 

  20. Radicella JP, Dherin C, Desmaze C et al. Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae. Proc Natl Acad Sci USA 1997; 94:8010–8015.

    Article  PubMed  CAS  Google Scholar 

  21. Rpldan-Arjona T, Wei YF, Carter KC et al. Molecular cloning and functional expression of a human cDNA encoding the antimutator enzyme 8-hydroxyguanine-DNA glycosylase. Proc Natl Acad Sci USA 1997; 94:8016–8020.

    Article  Google Scholar 

  22. Arai K, Morishita K, Shinmura K et al. Cloning of a human homolog of the yeast OGG1 gene that is involved in the repair of oxidative DNA damage. Oncogene 1997; 14:2857–2861.

    Article  PubMed  CAS  Google Scholar 

  23. Aburatani H, Hippo Y, Ishida T et al. Cloning and characterization of mammalian 8-hydroxyguanine-specific DNA glycosylase/apurinic, apyrimidinic lyase, a functional mutM homologue. Cancer Res 1997; 57:2151–2156.

    PubMed  CAS  Google Scholar 

  24. Hirano T, Kudo H, Doi Y et al. Detection of a smaller, 32-kDa 8-oxoguanine DNA glycosylase 1 in 3′-methyl-4-dimethylamino-azobenzene-treated mouse liver. Cancer Sci 2004; 95:118–122.

    Article  PubMed  CAS  Google Scholar 

  25. Hirano T, Kawai K, Ootsuyama Y et al. Detection of a mouse OGG1 fragment during caspase-dependent apoptosis: Oxidative DNA damage and apoptosis. Cancer Sci 2004; 95:634–638.

    Article  PubMed  CAS  Google Scholar 

  26. Tagesson C, Kallberg M, Klintenberg C et al. Determination of urinary 8-hydroxydeoxyguanosine by automated coupled-column high performance liquid chromatography: A powerful technique for assaying in vivo oxidative DNA damage in cancer patients. Eur J Cancer 1995; 31A:934–940.

    Article  PubMed  CAS  Google Scholar 

  27. Wu LL, Chiou CC, Chang PY et al. Urinary 8-OHdG: A marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta 2004; 339:1–9.

    Article  PubMed  CAS  Google Scholar 

  28. Gedik CM, Boyle SP, Wood SG et al. Oxidative stress in humans: Validation of biomarkers of DNA damage. Carcinogenesis 2002; 23:1441–1446.

    Article  PubMed  CAS  Google Scholar 

  29. Kasai H, Svoboda P, Yamazaki S. Simultaneous determination of 8-hydroxydeoxyguanosine, a marker of oxidative stress, and creatinine, a standardization compound, in urine. Industrial Health 2005; 43:333–336.

    Article  PubMed  CAS  Google Scholar 

  30. Svoboda P, Kasai H. Simultaneous HPLC analysis of 8-hydroxydeoxyguanosine and 7-methylguanine in urine from humans and rodents. Anal Biochem 2004; 334:239–250.

    Article  PubMed  CAS  Google Scholar 

  31. Irie M, Tamae K, Iwamoto-Tanaka N et al. Occupational and lifestyle factors and urinary 8-hydroxydeoxyguanosine. Cancer Sci 2005; 96:600–606.

    Article  PubMed  CAS  Google Scholar 

  32. Smythies J. Redox aspects of signaling by catecholamines and their metabolites. Antioxid Redox Signal 2000; 2:575–583.

    Article  PubMed  CAS  Google Scholar 

  33. Carsons S. The association of malignancy with rheumatic and connective tissue diseases. Semin Oncol 1997; 24:360–372.

    PubMed  CAS  Google Scholar 

  34. Kasai H, Hirano T, Tsurudome Y et al. Increase of urinary 8-OH-dG levels in cancer patients and cancer high-risk groups. Roles of Reactive Oxygen-and Nitrogen-species in Mutagenesis and Carcinogenesis. 8th International Conference on Environmental Mutagen, Nara Satellite Meeting, 82, (Abstract).

    Google Scholar 

  35. Ochi T, Takahashi K, Ohsawa M. Indirect evidence for the induction of a prooxidant state by cadmium chloride in cultures mammalian cells and a possible mechanism for the induction. Mutat Res 1987; 180:257–266.

    PubMed  CAS  Google Scholar 

  36. Bialkowski K, Bialkowska A, Kasprzak KS. Cadmium(II), unlike nickel(II), inhibits 8-oxo-dGTPase activity and increases 8-oxo-dG level in DNA of the rat testis, a target organ for cadmium(II) carcinogenesis. Carcinogenesis 1999; 20:1621–1624.

    Article  PubMed  CAS  Google Scholar 

  37. Wong RH, Yeh CY, Hsueh YM et al. Association of hepatitis virus infection, alcohol consumption and plasma vitamin A levels with urinary 8-hydroxydeoxyguanosine in chemical workers. Mutat Res 2003; 535:181–186.

    PubMed  CAS  Google Scholar 

  38. Hinokio Y, Suzuki S, Hirai M et al. Urinary excretion of 8-oxo-7, 8-dihydro-2′-deoxyguanosine as a predictor of the development of diabetic nephropathy. Diabetologia 2002; 45:877–882.

    Article  PubMed  CAS  Google Scholar 

  39. Nishikawa T, Sasahara T, Kiritoshi S et al. Evaluation of urinary 8-hydroxydeoxy-guanosine as a novel biomarker of macrovascular complications in type 2 diabetes. Diabetes Care 2003; 26:1507–1512.

    Article  PubMed  CAS  Google Scholar 

  40. Collins AR, Gedik CM, Olmedilla B et al. Oxidative DNA damage measured in human lymphocytes: Large differences between sexes and between countries, and correlations with heart disease mortality rates. FASEB J 1998; 12:1397–1400.

    PubMed  CAS  Google Scholar 

  41. Lovell MA, Markesbery WR. Ratio of 8-hydroxyguanine in intact DNA to free 8-hydroxyguanine is increased in Alzheimer disease ventricular cerebrospinal fluid. Arch Neurol 2001; 58:392–396.

    Article  PubMed  CAS  Google Scholar 

  42. Sato S, Mizuno Y, Hattori N. Urinary 8-hydroxydeoxyguanosine levels as a biomarker for progression of Parkinson disease. Neurology 2005; 64:1081–1083.

    PubMed  CAS  Google Scholar 

  43. Tsukahara H, Shibata R, Ohshima Y et al. Oxidative stress and altered antioxidant defenses in children with acute exacerbation of atopic dermatitis. Life Sci 2003; 72:2509=2516.

    Article  PubMed  Google Scholar 

  44. Matsubasa T, Uchino T, Karashima S et al. Oxidative stress in very low birth weight infants as measured by urinary 8-OHdG. Free Radic Res 2002; 36:189–193.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Departments of Environmental Oncology and Surgery I, University of Occupational and Environmental Health, Japan, 1-1 lseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Fukuoka, Japan

    H. Kasai, T. Hirano, K. Kawai, Y. Tsurudome & H. Itoh

  2. First Department of Internal Medicine, Kyushu University, Fukuoka City, Japan

    D. Himeji & T. Horiuchi

Authors
  1. H. Kasai
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  2. T. Hirano
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  3. K. Kawai
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  4. Y. Tsurudome
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  5. H. Itoh
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  6. D. Himeji
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  7. T. Horiuchi
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Editor information

Editors and Affiliations

  1. Radiation and Oxidative Stress Group Department of Cancer Studies and Molecular Medicine RKCSB, Leicester Royal Infirmary, University of Leicester, Leicester, UK

    Mark D. Evans B.Sc., Ph.D.

  2. Department of Cancer Studies and Molecular Medicine and Department of Genetics, University of Leicester, Leicester, UK

    Marcus S. Cooke B.Sc., M.Sc., Ph.D.

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© 2007 Landes Bioscience and Springer Science+Business Media

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Kasai, H. et al. (2007). Analysis of 8-Hydroxy-2′-Deoxyguanosine as a Marker of Oxidatively Damaged DNA in Relation to Carcinogenesis and Aging. In: Evans, M.D., Cooke, M.S. (eds) Oxidative Damage to Nucleic Acids. Molecular Biology Intelligence Unit. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72974-9_14

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