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Analysis of 8-Hydroxy-2′-Deoxyguanosine as a Marker of Oxidatively Damaged DNA in Relation to Carcinogenesis and Aging

  • H. Kasai
  • T. Hirano
  • K. Kawai
  • Y. Tsurudome
  • H. Itoh
  • D. Himeji
  • T. Horiuchi
Part of the Molecular Biology Intelligence Unit book series (MBIU)

Abstract

Reactive oxygen species (ROS) are well known hazards for living organisms and are believed to be associated with the induction of cancer. ROS induce many forms of oxidative damage to proteins, nucleic acids, and lipids. Therefore, to diagnose or prevent cancer, analyses of oxidative products in patient samples, including tissue, blood, and urine, are very informative. Amongst the products of DNA oxidation, 8-hydroxy-2′-deoxyguanosine (8-OH-dG) is an important form of damage that leads to point mutations in genomic DNA. Since 8-OH-dG is the most abundant form of oxidative DNA damage and is easy to detect in laboratories, using a high performance liquid chromatography (HPLC) system equipped with an electrochemical detector (ECD), many researchers studying cellular oxidative stress have primarily analyzed 8-OH-dG. In this chapter, we will describe our findings regarding 8-OH-dG generation and its repair, as well as our recent urinary 8-OH-dG data.

Keywords

High Performance Liquid Chromatography Atopic Dermatitis Diesel Exhaust Particle Cadmium Chloride Crocidolite Asbestos 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Landes Bioscience and Springer Science+Business Media 2007

Authors and Affiliations

  • H. Kasai
    • 1
  • T. Hirano
    • 1
  • K. Kawai
    • 1
  • Y. Tsurudome
    • 1
  • H. Itoh
    • 1
  • D. Himeji
    • 2
  • T. Horiuchi
    • 2
  1. 1.Departments of Environmental Oncology and Surgery IUniversity of Occupational and Environmental Health, JapanFukuokaJapan
  2. 2.First Department of Internal MedicineKyushu UniversityFukuoka CityJapan

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