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Microarrays pp 139–167Cite as

Peptide-Based Microarray

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Part of the book series: Integrated Analytical Systems ((ANASYS))

Abstract

The peptide array has come into focus as an emerging screening platform for large-scale protein detection and activity studies. The materials presented in this chapter examine the recent developments in the field of peptide microarrays with special emphasis on the generation and applications of high-density arrays of peptides on glass slides.

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Notes

  1. 1.

    An N-terminal cysteine or biotin may be added to virtually any synthetic peptide. This makes it possible to apply peptides synthesized from various combinatorial strategies, such as positional-scanning libraries, onto an array format. Within reasonable lengths, under 10 amino acid residues, Fmoc synthesis yields sufficiently pure peptides that may be directly applied to an array without extensive purification (also dependent on the nature of the residues within the peptide and quality of synthesis).

  2. 2.

    The carboxyl moiety of the coumarin was first activated by NHS/DCC/DIEA followed by direct spotting on the slides.

  3. 3.

    The presence of periodate and BSA in the reaction mix does not interfere with enzymatic activity but aids in the efficient oxidation sodium periodate oxidation of the 1, 2 diol/1, 2-aminoalcohol linker and the subsequent β-elimination.

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Authors and Affiliations

  1. Departments of Chemistry and Biological Sciences, Medicinal Chemistry Program of the Office of Life Sciences, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Republic of Singapore

    Resmi C. Panicker, Hongyan Sun, Grace Y. J. Chen & Shao Q. Yao

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  1. Resmi C. Panicker
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  2. Hongyan Sun
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  3. Grace Y. J. Chen
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  4. Shao Q. Yao
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Editors and Affiliations

  1. CombiMatrix Corporation, Inc. 6500 Harbour Heights Parkway, Suite 301, Mukilteo, WA, 98275, USA

    Kilian Dill

  2. Osmetech Molecular Diagnostics, 757 S. Raymond Ave., Pasadena, CA, 91105, USA

    Robin Hui Liu

  3. National Institutes of Health National Cancer Institute, 31 Center Drive, Bethesda, MD, 20892, USA

    Piotr Grodzinski

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Panicker, R.C., Sun, H., Chen, G.Y.J., Yao, S.Q. (2009). Peptide-Based Microarray. In: Dill, K., Liu, R.H., Grodzinski, P. (eds) Microarrays. Integrated Analytical Systems. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72719-6_7

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