Intensity Concentration Relationships for Electrochemical Detection
The currently accepted technology for polynucleotide quantitation on gene chips involves labelling the assayed polynucleotides with a fluorescent marker, hybridis ing the sample to the gene chip, and then imaging the gene chip under fluorescence. The background-corrected intensity of fluorescence is assumed to be proportional to the concentration of the target polynucleotide.
CombiMatrix have developed a novel technology for quantification of binding to their custom gene chip platform. The sensor is based on the horseradish peroxidase coupled electrochemical properties of each gene chip cell. The technology is inher ently cheaper, more reliable, more sensitive, and more robust than fluorescence based optical sensors of gene expression binding.
CombiMatrix have noted that the response between polynucleotide concentration and reading intensity is nonlinear. That is, there is no longer a simple proportional ity between background-corrected fluorescence and polynucleotide...
KeywordsBackground Correction Median Normalisation Gene Chip Principal Component Score Deconvolution Algorithm
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