Microarrays pp 25-34 | Cite as

Electrochemical Detection on Microarrays

  • Kilian Dill
  • Andrey Ghindilis
Part of the Integrated Analytical Systems book series (ANASYS)

2.1 Introduction

Microarray detection methods have long been based upon optical methods: visible detection, fluorescence, luminescence, and surface plasmon resonance (1, 2, 3, 4, 5, 6). In the case of the visible detection method an enzyme produces a substrate that forms an insoluble precipitate at the spot site, which becomes visible to the naked eye. Surface plasmon resonance also uses visible light, but in this case the energy (and reflected angle) of the light absorption on the metal surface is altered; the researcher finds the absorption minimum using an array detector. This method utilizes more expensive equipment in the process of detecting the material on the chip/slide (4).

More sensitive methods such as fluorescence and luminescence have also been employed for the detection of material on the chip/slide surface. The fluorescence-based system requires a laser, so the beam can be rastered over the chip area. Or conversely, the chip can be illuminated with light and then a...


Electrochemical Detection Capture Probe Microelectrode Array Redox Enzyme Duplex Formation 
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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Kilian Dill
    • 1
  • Andrey Ghindilis
    • 2
  1. 1.Independent ConsultantSultan
  2. 2.Sharp Labs of AmericaCamas

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