Electrochemical Detection on Microarrays
Microarray detection methods have long been based upon optical methods: visible detection, fluorescence, luminescence, and surface plasmon resonance (1, 2, 3, 4, 5, 6). In the case of the visible detection method an enzyme produces a substrate that forms an insoluble precipitate at the spot site, which becomes visible to the naked eye. Surface plasmon resonance also uses visible light, but in this case the energy (and reflected angle) of the light absorption on the metal surface is altered; the researcher finds the absorption minimum using an array detector. This method utilizes more expensive equipment in the process of detecting the material on the chip/slide (4).
More sensitive methods such as fluorescence and luminescence have also been employed for the detection of material on the chip/slide surface. The fluorescence-based system requires a laser, so the beam can be rastered over the chip area. Or conversely, the chip can be illuminated with light and then a...
KeywordsElectrochemical Detection Capture Probe Microelectrode Array Redox Enzyme Duplex Formation
- 18.Kerman K, Kobayashi M, Tamiya E, Recent trends in electrochemical DNA biosensor technology, Meas. Sci. Technol. 15: R1–R11, 204.Google Scholar
- 20.Guiducci C, Stagni C, Zuccheri G, Boglioli A, Benini L, Samori A, Ricco B, A biosensor for direct detection of DNA seqeunces based on capacitance measurements, 32nd European Solid-State Device Research Conference, Firenze, Italy, 2002.Google Scholar