Use of Redox Enzymes for the Electrochemical Detection of Sequence-Specific DNA and Immunochemical Entities
There is a host of methods for the detection of immunoassays and sequence-specific DNA on a microarray chip (or biochip); see earlier chapter. Many detection technologies are optical methods, such as surface plasmon resonance, luminescence, fluorescence, and visible detection modes (absorbance or reflectance). They do require optical systems that are somewhat expensive.
As shown in previous chapters, electrochemical detection is a viable option for immunochemical and sequence-specific DNA detection. The electrochemical detection methods vary greatly, with anything from impedance measurements, to oxidation of specific nucleotdides within the duplex, to conductive interacalators, redox-intercalators, metal tags, and redox enzyme systems. Many have specific niches and amplification modes. The bottom line is that electrochemical detection is sensitive, the system footprint is small, and the system is inexpensive.
To date only a few electrochemical detection systems have...
KeywordsElectrochemical Detection Electrode System Chip Surface Glucose Dehydrogenase Redox Enzyme
Acknowledgment Partial funding was provided by Phase II SBIR grant (DAAD13-00-C-0033).
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