eSensor®A Microarray Technology Based on Electrochemical Detection of Nucleic Acids and Its Application to Cystic Fibrosis Carrier Screening
We have developed a test for identification of carriers for cystic fibrosis using the eSensor® DNA detection technology. Oligonucleotide probes are deposited within self-assembled monolayers on gold electrodes arrayed upon printed circuit boards. These probes allow sequence-specific capture of amplicons containing a panel of mutation sites associated with cystic fibrosis. DNA targets are detected and mutations genotyped using a “sandwich” assay methodology employing electrochemical detection of ferrocene-labeled oligonucleotides for discrimination of carrier and non-carrier alleles. Performance of the cystic fibrosis application demonstrates sufficient accuracy and reliability for clinical diagnostic use, and the procedure can be performed by trained medical technologists available in the hospital laboratory.
KeywordsCystic Fibrosis Print Circuit Board Signal Probe Capture Probe Cystic Fibrosis Transmembrane Regulator
A large number of people have contributed to the development of the eSen-sor® DNA Detection Technology, beginning with the founders of Clinical Micro Sensors, Thomas Meade and Jon Faiz Kayyem, and including past employees of CMS and Motorola Life Sciences as well as current employees of Osmetech Molecular Diagnostics too numerous to mention by name, but whose creativity, enthusiasm, and hard work are gratefully acknowledged.
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