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Microarrays pp 247-260 | Cite as

eSensor®A Microarray Technology Based on Electrochemical Detection of Nucleic Acids and Its Application to Cystic Fibrosis Carrier Screening

  • Michael R. Reed
  • William A. Coty
Part of the Integrated Analytical Systems book series (ANASYS)

Abstract

We have developed a test for identification of carriers for cystic fibrosis using the eSensor® DNA detection technology. Oligonucleotide probes are deposited within self-assembled monolayers on gold electrodes arrayed upon printed circuit boards. These probes allow sequence-specific capture of amplicons containing a panel of mutation sites associated with cystic fibrosis. DNA targets are detected and mutations genotyped using a “sandwich” assay methodology employing electrochemical detection of ferrocene-labeled oligonucleotides for discrimination of carrier and non-carrier alleles. Performance of the cystic fibrosis application demonstrates sufficient accuracy and reliability for clinical diagnostic use, and the procedure can be performed by trained medical technologists available in the hospital laboratory.

Keywords

Cystic Fibrosis Print Circuit Board Signal Probe Capture Probe Cystic Fibrosis Transmembrane Regulator 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

A large number of people have contributed to the development of the eSen-sor® DNA Detection Technology, beginning with the founders of Clinical Micro Sensors, Thomas Meade and Jon Faiz Kayyem, and including past employees of CMS and Motorola Life Sciences as well as current employees of Osmetech Molecular Diagnostics too numerous to mention by name, but whose creativity, enthusiasm, and hard work are gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Michael R. Reed
    • 1
  • William A. Coty
    • 1
  1. 1.Osmetech Molecular DiagnosticsPasadena

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