New Approaches to the Synthesis of Addressable Microarray Molecular Libraries

Abstract

Approaches for the synthesis of molecular libraries on addressable arrays of microelectrodes are presented. In each case, substrates are fixed to a polymer coating of the array in the regions proximal to the microelectrodes. Selected micro-electrodes are then used to synthesize chemical reagents that initiate reactions involving the substrates. The reagents generated at the electrodes are confined to the region of the array next to the selected electrodes by placing a second substrate that consumes the reagent in the reaction solution above the array. In addition, a strategy for characterizing the molecules synthesized in this manner, and a strategy for probing their biological activity are described.