Expression Profiling Using Microfluidic Living Cell Arrays
10.1 Introduction to the Living Cell Array Concept
The cellular microenvironment is remarkably complex. In the small space near each cell, growth factors are liberated from extracellular matrix, cytokines are secreted by neighboring cells, and hormones arrive from distant endocrine organs through the circulation. These soluble cues are detected by surface or cytoplasmic receptors and integrated using complex signal transduction cascades to modulate the activity of transcription factors (TFs), the primary regulators of gene expression. Transcription factors serve as points of convergence between the vast number of extracellular signaling molecules and the equally vast number of target genes. For perspective, the human genome contains approximately 1500 identified transcription factors regulating more than 20,000 target genes .
Adding further complexity to the picture, transcription factors often cooperate, compete, and regulate each other, forming transcriptional regulatory networks...
KeywordsGreen Fluorescent Protein Microfluidic Device Transcriptional Regulatory Network Cell Array Master Mold
This work was supported in part by a Texas Engineering Experiment Station Award to AJ and grants from the NIH (BRP AI063795 and P41 EB002503) to MLY.
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