Abstract
Action spectroscopy is a method for identifying the light-absorbing chromophore in a photobiological or biochemical process by comparing the efficiency of radiation of different wavelengths in driving the process. This chapter explains the principle and gives several examples from the history of biology of what has been achieved using this technique.
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References
Avery, O.T., MacLeod, C.M. and McCarty, M. (1944) Studies on the chemical nature of the substance inducing transformation of pneumococcal types. J. Exp. Med. 79, 137–157.
Björn, L.O. (1969a) Action spectra for transformation and fluorescence of protochlorophyll holochrome from bean leaves. Physiol. Plant. 22, 1–17.
Björn, L.O. (1969b) Studies on the phototransformation and fluorescence of protochlorophyll holochrome in vitro. In H. Metzner (Ed.), Progress in photosynthesis research, Vol. II, pp. 618–629. H. Laupp Jr., Tübingen, Germany.
Björn, L.O., Sundqvist, C., and Öquist, G. (2007) A tribute to Per Halldal (1922–1986), a Norwegian photobiologist in Sweden. Photosynthesis Res. 92, 7–11.
Diakoff, S. and Scheibe, J. (1973) Action spectra for chromatic adaptation in Tolypothrix tenuis. Plant Physiol. 51, 382–385.
Engelmann, Th. W. (1882a) Ueber Sauerstoffausscheidung von Pflanzenzellen im Mikrospektrum. Bot. Ztg. 40, 419–426.
Engelmann, Th. W. (1882b) Ueber Assimilation von Haematococcus. Bot. Ztg. 40, 663–669.
Engelmann, Th. W. (1884) Untersuchungen über die quantitativen Beziehungen zwischen Absorption des Lichtes und Assimilation in Pflanzenzellen. Bot. Ztg. 42, 81–94, 97–106 and Tafel II.
Fujita, Y. and Hattori, A. (1962) Photochemical interconversion between precursors of phycobilin chromoproteids in Tolypothrix tenuis. Plant Cell Physiol. 3, 209–220.
Gates, F.L. (1928) On nuclear derivatives and the lethal action of ultra-violet light. Science 68, 479–480.
Gates, F.L. (1929) A study of the bactericidal action of ultra violet light. I. The reaction to monochromatic radiations. J. Gen. Physiol. 14, 31–42.
Gates, F.L. (1930). A study of the action of ultra violet light III. The absorption of ultra violet light by bacteria. J. Gen. Physiol. 14, 31–42.
Giese, A.C. and Leighton, P.A. (1935) Quantitative studies on the photolethal effects of quartz ultra-violet radiation upon Paramecium. J. Gen. Physiol. 18, 557–571.
Griffith, F. (1928) The significance of pneumococcal types. J. Hygiene 27, 113–159.
Hartmann, K.M. (1967) Ein Wirkungsspektrum der Photomorphogenese unter Hochenergiebedingungen und seine Interpretation auf der Basis des Phytochroms (Hypokotylwachstumshemmung bei Lactuca sativa L.). Z. Naturforsch. 22b, 266–275.
Haxo, F.T. (1960) The wavelength dependence of photosynthesis and the role of accessory pigments. In: M.B. Allen (Ed.), Comparative biochemistry of photoreactive systems, pp. 339–376. Academic Press, New York.
Haxo, F.T. and Blinks, L.R. (1950) Photosynthetic action spectra of marine algae. J. Gen. Physiol. 33, 389–422.
Hertel, E. (1905) Ueber physiologische Wirkung von Strahlen verschiedener Wellenlänge. Zschr. Allgem. Physiol. 5, 95–122.
Hollaender, A. and Claus, W.D. (1936) The bactericidal effect of ultraviolet radiation on Escherichia coli in liquid suspensions. J. Gen. Physiol. 19, 753–765.
Levring, T. (1947) Submarine daylight and the photosynthesis of marine algae. Göteborgs Kgl. Vetenskaps- och Vitterhets-samhälles Handl., 6:e följden, ser. B, band 5, nr 6. 90 s.
Pringsheim, N. (1886) Zur Beurtheilung der Engelmann’schen Bakterienmethode in ihrer Brauchbarkeit zur quantitativen Bestimmung der Sauerstoffabgabe im Spektrum. Berl. Deutsch. Bot. Ges. 4, 40–46.
Schopfer, P. and Siegelman, H.W. (1969) Purification of protochlorophyllide holochrome. In H. Metzner (Ed.), Progress in photosynthesis research, vol. II, pp. 612–618. H. Laupp Jr., Tübingen.
Sundqvist, C. and Björn, L.O. (2007) A tribute to Hemming Virgin, a Swedish pioneer in plant photobiology. Photosynth. Res. 92, 13–16.
Timiriazeff, C. (1885) Ètat actuel de nos conaissances sur la fonction chlorophyllienne. Ann. des Sc. Nat. Botanique (3) Tome II.
Vierstra, R.D. and Quail, P.H. (1983a) Purification and initial characterization of 124-kilodalton phytochrome from Avena. Biochemistry 22, 2498–2505.
Vierstra, R.D. and Quail, P.H. (1983b) Photochemistry of 124-kilodalton Avena phytochrome in vitro. Plant Physiol. 72, 264–267.
Vogelmann, T.C. and Scheibe, J. (1978) Action spectra for chromatic adaptation in the blue-green alga Fremyella diplosiphon. Planta 143, 233–239.
Warburg, O. (1926) Über die Wirkung des Kohlenoxyds auf den Stoffwechsel der Hefe. Biochem. Z. 177, 471–486.
Warburg, O. and Negelein, E. (1929a) bÜber die photochemische Dissoziation bei intermittierender Belichtung und das absolute Absorptionsspektrum des Atmungsferments. Biochem. Z. 202, 202–228.
Warburg, O. and Negelein, E. (1929b) Absolutes Absorptionsspektrum des Atmungsferments. Biochem. Z. 204, 495–499.
Withrow, R.B., Klein, W.H. and Elstad, V. (1957) Action spectra of photomorphogenetic induction and its inactivation. Plant Physiol. 32, 453–462.
Ziv, L., Tovin, A., Strasser, D. and Gothilf, Y. (2007) Spectral sensitivity of melatonin suppression in the zebrafish pineal gland. Exp. Eye Res. 84, 92–99.
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Björn, L.O. (2008). Action Spectroscopy in Biology. In: Björn, L.O. (eds) Photobiology. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72655-7_8
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DOI: https://doi.org/10.1007/978-0-387-72655-7_8
Publisher Name: Springer, New York, NY
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