Applications of Molecular Tests in Anatomic Pathology
Molecular testing in anatomic pathology is becoming increasingly important for most organ systems, including in the lung. Such tests are used both diagnostically and prognostically and are particularly important in the workup of neoplasia and for identification or subclassification for certain infectious processes. Fresh and frozen tissues are always considered to be the most optimal source of DNA and RNA that serves as the template for targeted molecular analysis. However, archival paraffin-embedded tissue is an attractive alternative source of tissue for clinical testing. Paraffin-embedded tissue can be a critical source of nucleic acid when unexpected diagnoses are rendered in the pathologic evaluation of tissue material. It also, however, provides the advantage of allowing for archival analysis with correlation to outcome.
KeywordsEpidermal Growth Factor Receptor Comparative Genomic Hybridization Epidermal Growth Factor Receptor Mutation KRAS Mutation Small Cell Carcinoma
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