Forward and Reverse Genetics in Soybean

  • Kristin D. Bilyeu
Part of the Plant Genetics and Genomics: Crops and Models book series (PGG, volume 2)


Phytic Acid Soybean Seed Reverse Genetic Soybean Line Linolenic Acid Level 
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  1. Aghoram, K., Wilson, R.F., Burton, J.W. and Dewey, R.E. (2006) A mutation in a 3-keto-acyl-ACP synthase II gene is associated with elevated palmitic acid levels in soybean seeds. Crop Sci. 46, 2453–2459.CrossRefGoogle Scholar
  2. Alt, J.L., Fehr, W.R., Welke, G.A. and Sandhu, D. (2005) Phenotypic and molecular analysis of oleate content in the mutant soybean line M23. Crop Sci. 45, 1997–2000.CrossRefGoogle Scholar
  3. Anai, T., Yamada, T., Kinoshita, T., Rahman, S.M. and Takagi, Y. (2005) Identification of corresponding genes for three low-alpha-linolenic acid mutants and elucidation of their contribution to fatty acid biosynthesis in soybean seed. Plant Sci. 168, 1615–1623.CrossRefGoogle Scholar
  4. Ashfield, T., Ong, L.E., Nobuta, K., Schneider, C.M. and Innes, R.W. (2004) Convergent evolution of disease resistance gene specificity in two flowering plant families. Plant Cell 16, 309–318.PubMedCrossRefGoogle Scholar
  5. Bhatia, C.R., Nichterlein, K. and Maluszynski, M. (1999) Oilseed cultivars developed from induced mutations and mutations altering fatty acid composition. Mutation Breeding Review 11, 1–36.Google Scholar
  6. Bilyeu, K.D., Palavalli, L., Sleper, D.A. and Beuselinck, P.R. (2003) Three microsomal omega-3 fatty-acid desaturase genes contribute to soybean linolenic acid levels. Crop Sci. 43, 1833–1838.Google Scholar
  7. Bilyeu, K., Palavalli, L., Sleper, D. and Beuselinck, P. (2005) Mutations in soybean microsomal omega-3 fatty acid desaturase genes reduce linolenic acid concentration in soybean seeds. Crop Sci. 45, 1830–1836.CrossRefGoogle Scholar
  8. Bilyeu, K., Palavalli, L., Sleper, D.A. and Beuselinck, P. (2006) Molecular genetic resources for development of 1% linolenic acid soybeans. Crop Sci. 46, 1913–1918.CrossRefGoogle Scholar
  9. Chappell, A.S. and Bilyeu, K.D. (2006) A GmFAD3A mutation in the low linolenic acid soybean mutant C1640. Plant Breeding 125, 535–536.CrossRefGoogle Scholar
  10. Colbert, T., Till, B.J., Tompa, R., Reynolds, S., Steine, M.N., Yeung, A.T., McCallum, C.M., Comai, L. and Henikoff, S. (2001) High-throughput screening for induced point mutations. Plant Phys. 126, 480–484.CrossRefGoogle Scholar
  11. Gao, H., Narayanan, N.N., Ellison, L. and Bhattacharyya, M.K. (2005) Two classes of highly similar coiled coil-nucleotide binding-leucine rich repeat genes isolated from the rps1-k locus encode phytophthora resistance in soybean. Mol. Plant. Micr. Int. 18, 1035–1045.CrossRefGoogle Scholar
  12. Hitz, W.D., Carlson, T.J., Kerr, P.S. and Sebastian, S.A. (2002) Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds. Plant Physiol. 128, 650–660.PubMedCrossRefGoogle Scholar
  13. Kolkman, J.M., Conrad, L.J., Farmer, P.R., Hardeman, K., Ahern, K.R., Lewis, P.E., Sawers, R.J.H., Lebejko, S., Chomet, P. and Brutnell, T.P. (2005) Distribution of activator (ac) throughout the maize genome for use in regional mutagenesis. Genetics 169, 981–995.PubMedCrossRefGoogle Scholar
  14. Krysan, P.J., Young, J.C. and Sussman, M.R. (1999) T-DNA as an insertional mutagen in Arabidopsis. Plant Cell 11, 2283–2290.PubMedCrossRefGoogle Scholar
  15. Men, A.E., Laniya, T.S., Searle, I.R., Iturbe-Ormaetxe, I., Gresshoff, I., Jiang, Q., Carroll, B.J. and Gresshoff, P.M. (2002) Fast neutron mutagenesis of soybean (Glycine soja l.) produces a supernodulating mutant containing a large deletion in linkage group H. Genome Letters 1, 147–155.CrossRefGoogle Scholar
  16. Muskett, P.R., Clissold, L., Marocco, A., Springer, P.S., Martienssen, R. and Dean, C. (2003) A resource of mapped dissociation launch pads for targeted insertional mutagenesis in the Arabidopsis genome. Plant Physiol. 132, 506–516.PubMedCrossRefGoogle Scholar
  17. Palmer, R.G., Pfeiffer, T.W., Buss, G.R. and Kilen, T.C. (2004) Qualitative genetics. In: J.E. Specht and H.R. Boerma (Eds.), Soybeans: Improvement, Production, and Uses, Crop Sci. Society of America Monograph, Madison, WI, pp. 137–234.Google Scholar
  18. Rahman, S.M., Takagi, Y., Kubota, K., Miyamoto, K. and Kawakita, T. (1994) High oleic acid mutant in soybean induced by X-ray irradiation. Biosci. Biotech. Biochem. 58, 1070–1072.Google Scholar
  19. Rahman, S.M., Takagi, Y., Miyamoto, K. and Kawakita, T. (1995) High stearic acid soybean mutant induced by X-ray irradiation. Biosci. Biotech. Biochem.59, 922–926.Google Scholar
  20. Rajcan, I., Hou, G.Y. and Weir, A.D. (2005) Advances in breeding of seed-quality traits in soybean J. Crop Improvement 14, 145–174.CrossRefGoogle Scholar
  21. Ross, A.J., Fehr, W.R., Welke, G.A. and Cianzio, S.R. (2000) Agronomic and seed traits of 1%-linolenate soybean genotypes. Crop Sci. 40, 383–386.Google Scholar
  22. Sallaud, C., Gay, C., Larmande, P., Bes, M., Piffanelli, P., Piegu, B., Droc, G., Regad, F., Bourgeois, E., Meynard, D., Perin, C., Sabau, X., Ghesquiere, A., Glaszmann, J.C., Delseny, M. and Guiderdoni, E. (2004) High throughput T-DNA insertion mutagenesis in rice: A first step towards in silico reverse genetics. The Plant J. 39, 450–464.CrossRefGoogle Scholar
  23. Searle, I.R., Men, A.E., Laniya, T.S., Buzas, D.M., Iturbe-Ormaetxe, I., Carroll, B.J. and Gresshoff, P.M. (2003) Long-distance signaling in nodulation directed by a clavata1-like receptor kinase. Science 299, 109–112.PubMedCrossRefGoogle Scholar
  24. Stojsin, D., Luzzi, B.M., Ablett, G.R. and Tanner, J.W. (1998) Inheritance of low linolenic acid level in the soybean line RG10. Crop Sci. 38, 1441–1444.Google Scholar
  25. Takagi, Y., Hossain, A.B.M.M., Yanagita, T., Matsueda, T. and Murayama, A. (1990) Linolenic acid content in soybean improved by X-ray irradiation. Agric. Biol. Chem 54, 1735–1738.Google Scholar
  26. Takagi, Y., Rahman, S.M., Anai, T., Wasala, S.K., Kinoshita, T. and Khalekuzzaman, M. (1999) Development of a reduced linolenate soy mutant by re-irradiation and its genetic analysis. Breeding Science 49, 1–5.Google Scholar
  27. Takagi, Y., Rahman, S.M., Joo, H. and Kawakita, T. (1995) Reduced and elevated palmitic acid mutants in soybean developed by X-ray irradiation. Biosci. Biotech. Biochem.59, 1778–1779.CrossRefGoogle Scholar
  28. Walbot, V. (2000) Saturation mutagenesis using maize transposons. Curr. Op. Plant Bio. 3, 103–107.CrossRefGoogle Scholar
  29. Walker, D.R., Scaboo, A.M., Pantalone, V.R., Wilcox, J.R. and Boerma, H.R. (2006) Genetic mapping of loci associated with seed phytic acid content in CX1834-1-2 soybean. Crop Sci. 46, 390–397.CrossRefGoogle Scholar
  30. Wilcox, J.R., Premachandra, G.S., Young, K.A. and Raboy, V. (2000) Isolation of high seed inorganic P, low-phytate soybean mutants. Crop Sci. 40, 1601–1605.Google Scholar
  31. Yadav, N.S., Wierzbicki, A., Aegerter, M., Caster, C.S., Perez-Grau, L., Kinney, A.J., Hitz, W.D., Booth, J.R., Jr., Schweiger, B., Stecca, K.L., Allen, S.M., Blackwell, M., Reiter, R.S., Carlson, T.J., Russell, S.H., Feldmann, K.A., Pierce, J. and Browse, J. (1993) Cloning of higher plant omega-3 fatty acid desaturases. Plant Physiol. 103, 467–76.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Kristin D. Bilyeu
    • 1
  1. 1.USDA-ARS, Plant Genetics Research UnitUniversity of Missouri-ColumbiaUSA

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