Only microalgae (algae with cell/filament/thallus size <2 mm) are considered in this chapter. However, the methods described are applicable to a wide variety of prokaryotic cyanobacteria and eukaryotic microalgae. Most culturable cyanobacteria and soil microalgae that have been examined can be cryopreserved with relatively high viability. Furthermore, many freshwater and marine eukaryotic algae can also be cryopreserved, but usually with lower viability. Marine diatoms can be cryopreserved, and often have high viability (Day and Brand 2005), although there has been limited success preserving freshwater diatoms (McLellan 1989).
It has been observed that chlorarachniophytes, eustigmatophytes, pelagophytes, phaeothamniophytes and ulvophytes often have very high success rates, comparable to the other green algae and cyanobacteria (Day and Brand 2005). However, to date, most morphologically complex taxa including members of the dinoflagellates, cryptophytes, synurophytes and raphidophytes cannot be successfully cryopreserved. It is anticipated that further research on the basic mechanisms of freezing damage and the empirical development of improved protocols will continue to expand the number and diversity of algal taxa responsive to cryopreservation techniques.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Beaty MH, Parker BC (1992) Cryopreservation of eukaryotic algae. VA J Sci 43: 403-410.
Ben-Amotz A, Gilboa A (1980) Cryopreservation of marine unicellular algae. II. Induction of freezing tolerance. Mar Ecol Prog Series 2: 221-224.
Bodas K, Brennig C, Diller KR, Brand JJ (1995) Cryopreservation of blue-green and eukaryotic algae in the culture collection at the University of Texas at Austin. CryoLetters 16: 267-274.
Brand JJ, Diller KR (2004) Principles and applications of algal cryopreservation. Nova Hedwigia 79: 175-190.
Cañavate JP, Lubian LM (1995) Relationship between cooling rates, cryoprotectant concentrations and salinates in the cryopreservation of marine microalgae. Mar Biol 124: 325-334.
Cañavate JP, Lubian LM (1997) Effect of culture age on cryopreservation of marine microalgae. Eur J Phycol 32: 87-90.
Crutchfield ALM, Diller KR, Brand JJ (1999) Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta). Eur J Phycol 34: 43-52.
Day JG (1998) Cryoconservation of microalgae and cyanobacteria. CryoLetters Suppl 1: 7-14.
Day JG, Brand JJ (2005) Cryopreservation methods for maintaining cultures. In: Andersen RA (ed.) Algal Culturing Techniques. Academic Press, New York, pp. 165-187.
Day JG, De Ville MM (1995) Cryopreservation of algae. In: Day JG, McLellan MR (eds.) Methods in Molecular Biology. Vol. 38: Cryopreservation and Freeze-Drying Protocols, Hunama Press Inc., Totowa, NJ, pp. 81-88.
Day JG, Fenwick C (1993) Cryopreservation of members of the genus Tetraselmis used in aquaculture. Aquaculture 118: 151-160.
Day JG, Fleck RA, Benson EE (2000) Cryopreservation-recalcitrance in microalgae: Novel approaches to identify and avoid cryo-injury. J Appl Phycol 12: 369-377.
Day JG, Benson EE, Harding K, Knowles B, Idowu M, Bremner D, Santos L, Santos F, Friedl T, Lorenz M, Lukesova A, Elster J, Lukavsky J, Herdman M, Rippka R, Hall T (2005) Cryopreservation and conservation of microalgae: The development of a pan-European scientific and biotechnological resource (the COBRA project). CryoLetters 26: 231-238.
Fabre J, Dereuddre J (1990) Encapsulation/dehydration a new approach to cryopreservation of Solanum shoot-tips. CryoLetters 11: 413-426.
Fenwick C, Day JG (1992) Cryopreservation of Tetraselmis suecica cultured under different nutrients regimes. J Appl Phycol 4: 105-109.
Fleck RA (1998) The assessment of cell damage and recovery in cryopreserved freshwater protists. PhD Thesis, University Abertay Dundee, pp. 393.
Fleck RA, Day JG, Rana KJ, Benson EE (1997) Visualization of cryoinjury and freeze events in the coenocytic alga Vaucheria sessilis using cryomicroscopy. CryoLetters 18: 343-355.
Harding K, Day JG, Lorenz M, Timmermann H, Friedl T, Bremner DH, Benson EE (2004) Introducing the concept and application of vitrification for the cryo-conservation of algae—a mini review. Nova Hedwigia 79: 207-226.
McLellan MR (1989) Cryopreservation of diatoms. Diatom Res 4: 301-318.
Morris GJ (1976a) The cryopreservation of Chlorella 1. Interactions of rate of cooling, protective additive and warming rate. Arch Microbiol 107: 57-62.
Morris GJ (1976b) The cryopreservation of Chlorella 2. Effect of growth temperature on freezing tolerance. Arch Microbiol 107: 309-312.
Morris GJ (1978) Cryopreservation of 250 strains of Chlorococcales by the method of two step cooling. Br Phycol J 13: 15-24.
Morris GJ, Clarke KJ, Clarke A (1977) The cryopreservation of Chlorella 3. Effect of heterotrophic nutrition on freezing tolerance. Arch Microbiol 114: 249-254.
Starr RC, Zeikus JA (1993) UTEX—The culture collection of algae at the University of Texas at Austin. J Phycol Suppl 29: 1-106.
Taylor R, Fletcher RL (1998) Cryopreservation of eukaryotic algae—a review of methodologies. J Appl Phycol 10: 481-501.
Watanabe MM, Shimizu A, Satake K (1992) NIES-Microbial Culture Collection at the National Institute of Environmental Studies: Cryopreservation and database of culture strains of microalgae. In: Watanabe MM (ed.) Proceedings of Symposium on Culture Collection of Algae. NIES, Tsukuba, Japan, pp. 33-41.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer Science+Business Media, LLC
About this chapter
Cite this chapter
Day, J.G., Harding, K. (2008). Cryopreservation of Algae. In: Reed, B.M. (eds) Plant Cryopreservation: A Practical Guide. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72276-4_6
Download citation
DOI: https://doi.org/10.1007/978-0-387-72276-4_6
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-72275-7
Online ISBN: 978-0-387-72276-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)