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Cryopreservation of Temperate Berry Crops

  • Barbara M. Reed

Preservation of clonal genotypes of most fruit crops requires vegetative propagation, so cryopreservation is an ideal method for long-term germplasm storage (Engelmann 2004). Some of the first and most successful experiments with the cryopreservation of apical meristems began with a temperate berry crop, strawberry (Kartha et al. 1980; Sakai et al. 1978). These initial studies showed that slow cooling of <1 mm shoot tips in cryoprotectant solutions was a feasible option for long-term storage of clonally propagated plants. During the last two decades, cryopreservation methods were developed for many species of fruit crops (Reed 2001).

Keywords

Cold Acclimation Sterile Filter Paper Calcium Chloride Solution Recovery Medium Benzyl Amino Purine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Barbara M. Reed
    • 1
  1. 1.US Department of Agriculture-Agricultural Research ServiceNational Clonal Germplasm RepositoryCorvallisUSA

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