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Cryopreservation of Herbaceous Dicots

  • E. R. Joachim Keller
  • Angelika Senula
  • Anja Kaczmarczyk

In tissue culture herbaceous dicots play an important role as model species for various fundamental questions such as somatic embryogenesis (carrot, alfalfa), hormone interrelationships (tobacco), and anther culture (tobacco and Datura). This may be facilitated by high plasticity in some groups like the Solanaceae. Crops that must be maintained vegetatively are candidates for cryopreservation. Many important species belong to this group of herbaceous dicots: potato, cassava, and sweet potato, staple foods in many parts of the world, medicinal, and aromatic plants like mint, foxglove, chamomile and St. John’s Wort and ornamentals such as Chrysanthemum, Pelargonium, Dianthus, and Gentiana. In contrast to monocots which hide their meristems in the basal inner part of the shoot or bulb, most dicots present exposed shoot buds making their isolation easier and enabling the lab workers to more easily collect explants for cryopreservation. With respect to the other factors that influence cryopreservation, herbaceous dicots are as diverse as other plant groups, and generalization is difficult.

Keywords

Cold Acclimation Alginate Solution Vitrification Solution Recovery Medium Sterile Pipette 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • E. R. Joachim Keller
    • 1
  • Angelika Senula
    • 1
  • Anja Kaczmarczyk
    • 1
  1. 1.Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)Germany

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