The Discovery of Multiple Serotonin Receptor Subtypes: A Lesson from Molecular Cloning to Functional Expression
The field of serotonin (5-HT) receptor pharmacology has been at least as dramatically altered by the advent of molecular neurobiology and recombinant DNA techniques, as has any other neurotransmitter receptor field. The principal reason for this is the unforeseen multitude of genes expressing different types of 5-HT receptors. Classical pharmacological studies as well as radioligand-binding studies convinced workers in the field that there were multiple 5-HT receptors. The extent of that multiplicity was not generally foreseen. Thirteen 5-HT receptor genes have been cloned and functionally expressed. All 13 receptors were cloned between the years 1987 and 1995. Adding to the complexity of 5-HT receptors pharmacology are the splice variants and, in one case, mRNA editing isoforms, detected in the last decade. Bringing order to this rapid outpouring of information at this time is a very difficult task. In order to provide a concise and timely review, this article focuses on the strategies used to clone and express multiple 5-HT receptors. Unique properties of the various receptors are emphasized.
KeywordsIrritable Bowel Syndrome Adenylate Cyclase Xenopus Oocyte Serotonin Receptor Functional Expression
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