Oral Vaccination with Different Antigens from Yersinia pestis KIM Delivered by Live Attenuated Salmonella Typhimurium Elicits a Protective Immune Response Against Plague

  • Christine G. Branger
  • Roy Curtiss III
  • Robert D. Perry
  • Jacqueline D. Fetherston
Part of the Advances In Experimental Medicine And Biology book series (AEMB, volume 603)

The use of live recombinant Salmonella attenuated vaccine (RASV) encoding Yersinia proteins is a promising new approach for the vaccination against Yersinia pestis. We have tested the efficacy of 2 proteins, Psn and a portion of LcrV in protecting mice against virulent Yersinia pestis challenge. To remove the immunosuppressive properties of LcrV protein, the lcrV gene, without the TLR2 receptor sequence, was cloned into a β-lactamase secretion vector. Immunizations were performed with RSAV expressing LcrV or Psn. Challenge with a virulent Y. pestis strain was performed 4 weeks after the last immunization. Our results show that the truncated LcrV protein delivered by RASV is sufficient to afford a full protective immune response in a mouse model of bubonic plague and the Psn protein afforded partial protection in a non-optimized system. This finding should facilitate the design and development of a new generation of vaccines against Y. pestis.


Challenge Dose Yersinia Pestis Empty Plasmid Oral Immunization Oral Vaccination 
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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Christine G. Branger
    • 1
  • Roy Curtiss III
    • 1
  • Robert D. Perry
    • 2
  • Jacqueline D. Fetherston
    • 2
  1. 1.Center for Infectious Diseases and VaccinologyThe Biodesign Institute, Arizona State UniversityTempeUSA
  2. 2.Department of Microbiology, Immunology and Molecular GeneticsUniversity of KentuckyLexingtonUSA

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