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Oral Vaccination with Different Antigens from Yersinia pestis KIM Delivered by Live Attenuated Salmonella Typhimurium Elicits a Protective Immune Response Against Plague

  • Christine G. Branger
  • Roy Curtiss III
  • Robert D. Perry
  • Jacqueline D. Fetherston
Part of the Advances In Experimental Medicine And Biology book series (AEMB, volume 603)

The use of live recombinant Salmonella attenuated vaccine (RASV) encoding Yersinia proteins is a promising new approach for the vaccination against Yersinia pestis. We have tested the efficacy of 2 proteins, Psn and a portion of LcrV in protecting mice against virulent Yersinia pestis challenge. To remove the immunosuppressive properties of LcrV protein, the lcrV gene, without the TLR2 receptor sequence, was cloned into a β-lactamase secretion vector. Immunizations were performed with RSAV expressing LcrV or Psn. Challenge with a virulent Y. pestis strain was performed 4 weeks after the last immunization. Our results show that the truncated LcrV protein delivered by RASV is sufficient to afford a full protective immune response in a mouse model of bubonic plague and the Psn protein afforded partial protection in a non-optimized system. This finding should facilitate the design and development of a new generation of vaccines against Y. pestis.

Keywords

Challenge Dose Yersinia Pestis Empty Plasmid Oral Immunization Oral Vaccination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Christine G. Branger
    • 1
  • Roy Curtiss III
    • 1
  • Robert D. Perry
    • 2
  • Jacqueline D. Fetherston
    • 2
  1. 1.Center for Infectious Diseases and VaccinologyThe Biodesign Institute, Arizona State UniversityTempeUSA
  2. 2.Department of Microbiology, Immunology and Molecular GeneticsUniversity of KentuckyLexingtonUSA

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