Advertisement

Enrichment of Yersinia pestis from Blood Cultures Enables Rapid Antimicrobial Susceptibility Determination by Flow Cytometry

  • Raphael Ber
  • Moshe Aftalion
  • Sara Cohen
  • Yehuda Flashner
  • Emanuelle Mamroud
  • David Gur
  • Ida Steinberger-Levy
  • Eran Zahavy
Part of the Advances In Experimental Medicine And Biology book series (AEMB, volume 603)

Mortality from plague is high if not treated with the proper antibiotics within 18-24 hours after onset of symptoms. The process of antibiotic susceptibility determination of Yersinia pestis isolated from blood samples may extend from 4 to more than 7 days, since the in vitro growth is very slow. To accelerate this process, we developed an enrichment protocol as well as a non-standard yet reliable method for rapid antibiotic susceptibility analysis of Y. pestis from blood cultures using flow cytometry technology. This rapid method is applicable to blood cultures containing low levels of Y. pestis.

Keywords

Blood Culture Antimicrobial Susceptibility Blood Component Bacterial Concentration Yersinia Pestis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alvarez-Barrientons, A., Arroyo, J., Canton, R., Nombela, C. and Sanchez-Perez, M. (2000) Application of flow cytometry to clinical microbiology. Clin. Microbiol. Rev. 13, 167-195.CrossRefGoogle Scholar
  2. Ber, R., Mamroud, E., Aftalion, M., Tidhar, A., Gur, D., Flashner, Y. and Cohen, S. (2003) Development of an improved selective agar medium for isolation of Yersinia pestis. Appl. Environ. Microbiol. 69, 5787-5792.CrossRefPubMedPubMedCentralGoogle Scholar
  3. Butler, T., Levine, J., Linh, N., Chau, D., Adickman, M. and Arnold, K. (1976) Yersinia pestis infection in Vietnam. II: Quantitative blood cultures and detection of endotoxin in the cerebrospinal fluid of patients with meningitis. J. Infect. Dis. 133, 493-498.CrossRefPubMedGoogle Scholar
  4. Butler, T. (1983) Plague and other Yersinia infections. In: Greenough W. B. III and Merigan T. C. (Eds.), Current topics in infectious disease. Plenum Medical Book and Company, New York.Google Scholar
  5. CDC (2000) Antimicrobial susceptibility tests for Yersinia pestis. In: Laboratory manual of plague diagnostic tests. pp. 34-38.Google Scholar
  6. CLSI (2006) Performance standards for antimicrobial susceptibility testing: sixteenth informa-tional supplement. M100-S16.Google Scholar
  7. Flashner, Y., Mamroud, E., Tidhar, A., Ber, R., Aftalion, M., Gur, D., Lazar, S., Zvi, A., Bino, T., Ariel, N., Velan, B., Shafferman, A. and Cohen, S. (2004) Generation of Yersinia pestis attenuated strains by signature-tagged mutagenesis in search of novel vaccine candidates. Infect. Immun. 72, 908-915.CrossRefPubMedPubMedCentralGoogle Scholar
  8. Inglesby, T., Dennis, D., Henderson, D., Bartlett, J., Ascher, M., Eitzen, E., Fine, A., Fried-lander, A., Hauer, J., Koerner, J., Layton, M., McDade, J., Osterholm, M., O’Toole, T., Parker, G., Perl, T., Russell, P., Schoch-Spana, M. and Tonat, K. (2000) Plague as biologi-cal weapon- Medical and public health management. J. of American Medical Association. 283, 2281-2290.CrossRefGoogle Scholar
  9. Mansour, J., Robson, J., Arndt, C. and Schulte, T. (1985) Detection of Eschcerichia coli in blood using flow cytometry. Cytometry 6, 186-190.CrossRefPubMedGoogle Scholar
  10. Perry, R. and Fetherston, J. (1997) Yersinia pestis- Etiologic agent of plague. Clin. Microbiol. Rev. 10, 35-66.PubMedPubMedCentralGoogle Scholar
  11. Pollitzer, R. (1954) Plague. WHO Monograph series 22. WHO, Geneva, Swizerland.Google Scholar
  12. Steen, H. (2000) Flow cytometry of bacteria: glimps from past with a view to the future. J. Microb. Methods. 42, 65-74.CrossRefGoogle Scholar
  13. WHO (1999) Treatment of Plague. In: Plague manual: Epidemiology, distribution, surveil-lance and control. pp. 55-62.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Raphael Ber
    • 1
  • Moshe Aftalion
    • 1
  • Sara Cohen
    • 1
  • Yehuda Flashner
    • 1
  • Emanuelle Mamroud
    • 1
  • David Gur
    • 1
  • Ida Steinberger-Levy
    • 1
  • Eran Zahavy
    • 2
  1. 1.Department of Biochemistry and Molecular GeneticsIsrael Institute for Biological ResearchIsrael
  2. 2.Department of Infectious DiseasesIsrael Institute for Biological ResearchIsrael

Personalised recommendations