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3 IS-RFLP: A Powerful Tool for Geographical Clustering of Global Isolates of Yersinia pestis

  • Alexandre J. L. Leclercq
  • Elisabeth Carniel
  • Viviane Chenal-Francisque
  • Gabriela Torrea
Part of the Advances In Experimental Medicine And Biology book series (AEMB, volume 603)

Multiple copies of several classes of insertion sequences (IS) are found in the genome of Yersinia pestis, the causative agent of bubonic and pneumonic plague. We used the genetic instability generated by these IS to develop a method (designated 3IS-RFLP) based on the restriction fragment length polymorphism of the IS100, IS285 and IS1541 elements for studying Y. pestis strains of worldwide origin. We show that 3IS-RFLP is a powerful tool to group Y. pestis isolates according to their geographical origin, and therefore that this method may be valuable for investigating the origin of new or re-emerging plague foci or for addressing forensic issues.

Keywords

Insertion Sequence Geographical Cluster Yersinia Pestis IS1541 Element Pestis Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Alexandre J. L. Leclercq
    • 1
  • Elisabeth Carniel
    • 1
  • Viviane Chenal-Francisque
    • 1
  • Gabriela Torrea
    • 1
  1. 1.Yersinia Research UnitNational Reference Laboratory and WHO Collaborative Center for Yersinia, Institut PasteurFrance

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