Polynucleotide Phosphorylase and the T3SS

  • Jason A. Rosenzweig
  • Kurt Schesser
Part of the Advances In Experimental Medicine And Biology book series (AEMB, volume 603)

Low temperatures as well as encounters with host phagocytes are two stresses that have been relatively well studied in many species of bacteria. The exoribonuclease polynucleotide phosphorylase (PNPase) has previously been shown to be required by several species of bacteria, including Yersinia, for low-temperature growth. We have shown that PNPase also enhances the ability of Yersinia to withstand the killing activities of murine macrophages. We have gone on to show that PNPase is required for the optimal functioning of Yersinia’s type three secretion system (T3SS), an organelle that injects effector proteins directly into host cells. Surprisingly, the PNPase-mediated effect on T3SS activity is independent of PNPase’s ribonuclease activity and instead requires only its S1 RNA-binding domain. In stark contrast, the catalytic activity of PNPase is strictly required for enhanced growth at low temperature. Preliminary experiments suggest that the RNA-binding interface of the S1 domain is critical for its T3SS-enhancing activity. Our findings indicate that PNPase plays versatile roles in promoting Yersinia’s survival in response to stressful conditions.


Yersinia Enterocolitica Yersinia Pestis Virulence Plasmid Yersinia Species Yersinia Pseudotuberculosis 
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© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Jason A. Rosenzweig
    • 1
  • Kurt Schesser
    • 2
  1. 1.Division of Math Science and TechnologyNova Southeastern UniversityUSA
  2. 2.Department of Microbiology & Immunology, Miller School of MedicineUniversity of MiamiMiamiUSA

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