Functional Quorum Sensing Systems Affect Biofilm Formation and Protein Expression in Yersinia pestis

  • Alexander G. Bobrov
  • Arwa Abu Khweek
  • Robert D. Perry
  • Kenneth D. Parrish
  • Jacqueline D. Fetherston
  • Scott W. Bearden
Part of the Advances In Experimental Medicine And Biology book series (AEMB, volume 603)

Gram-negative bacteria predominantly use two types of quorum sensing (QS) systems - LuxI-LuxR, responsible for synthesis of N-acylhomoserine lactones (AHL or AI-1 signal molecule), and LuxS, which makes furanones (AI-2 signal molecule). We showed that LuxS and two LuxI-LuxR (YtbIR and YpsIR) systems are functional in Y. pestis. Four different AHL molecules were detected in Y. pestis extracts using TLC bioassays. Our data suggest that YtbIR is responsible for the production of long chain AHLs. Confocal laser scanning microscopy showed that biofilm formation is decreased in an ytbIR ypsIR luxS mutant. Twodimensional gel electrophoresis revealed altered levels of protein expression in a Y. pestis triple QS mutant at 26°C and 37°C.


Quorum Sense Luria Broth lacZ Reporter Yersinia Pestis Pestis Strain 
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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Alexander G. Bobrov
    • 1
  • Arwa Abu Khweek
    • 1
  • Robert D. Perry
    • 1
  • Kenneth D. Parrish
    • 2
  • Jacqueline D. Fetherston
    • 1
  • Scott W. Bearden
    • 3
  1. 1.Department of Microbiology, Immunology and Molecular GeneticsUniversity of KentuckyLexingtonUSA
  2. 2.Advanced Implant & Perio CenterLouisvilleUSA
  3. 3.Bacterial Zoonoses BranchCenters for Disease Control and PreventionFort CollinsUSA

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