Abstract
The necessity of probe validation of widely used iron oxide particles in molecular magnetic resonance imaging (mMRI) has called for a growing demand for simple detection and quantification methods in vitro and ex vivo. A simple option to adapt these particles to standard methods of biological research such as microscopy and flow cytometry is the conjugation of fluorescent dyes. With the rapid developing optical imaging techniques such magnetoptical probes could not only be used for mMRI, but also for in vivo fluorescence imaging. Furthermore, the option of screening magnetoptical particles for their binding affinity to target cells has been employed to optimize particle designs or to screen libraries of particles with unknown binding properties for their binding affinity to target cells. Finally, the conjugation of fluorescence dyes via specifically cleavable linkers to MR detectable nanoparticles provides the option to image probe distribution by MRI and single or even multiple enzyme functions by fluorescence imaging.
Thus, fluorescent dyes do not only add functionality for improving probes for mMRI, but also increase potential information content by enabling multi-modal imaging methods.
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Schellenberger, E. (2008). Magnetoptical Probes. In: Bulte, J.W., Modo, M.M. (eds) Nanoparticles in Biomedical Imaging. Fundamental Biomedical Technologies, vol 102. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72027-2_10
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DOI: https://doi.org/10.1007/978-0-387-72027-2_10
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