Evaluation of Bactericidal Activity of Human Biological Fluids by Flow Cytofluorimetry

  • Anna Budikhina
  • Boris Pinegin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 601)


The ability of biological fluids to kill microbes is an important feature of the human immune system. Following incubation of fluorescein isothiocyanate-labeled Staphylococcus aureus with biological specimens and subsequent staining with propidium iodide, the proportions of killed bacteria were estimated by flow cytometry. FACScan is a simple, quick and reliable method to evaluate bactericidal activity of biological fluids. The results of the cytometric method correlated well with the results of the classic microbiological method. The proposed method is highly informative for evaluating bactericidal activity of sera in immunocompromised patients.


Bactericidal Activity Multiple Myeloma Healthy Donor Biological Fluid Human Immune System 
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  1. Ahmad, M., Piludu, M., Oppenheim, F.G., Helmerhorst, E.J. and Hand, A.R. (2004) Immunocytochemical localization of histatins in human salivary glands. J. Histochem. Cytochem. Pharmacol. 52, 361–370.Google Scholar
  2. Caccavo, D, Pellegrino, N.M., Altamura, M, Rigon, A, Amati, L, Amoroso, A. and Jirillo, E. (2002) Antimicrobial and immunoregulatory functions of lactoferrin and its potential therapeutic application. J. Endotoxin Res. 8, 403–417.PubMedGoogle Scholar
  3. Fierer, J, Finley, F. and Braude, A.I. (1974) Release of C51-endotoxin from bacteria as assay of serum bactericidal activity. J. Immunol. 112, 2184–2192.PubMedGoogle Scholar
  4. Ginsburg, I. (2004) Bactericidal cationic peptides can also function as bacteriolysis-inducting agents mimicking beta-lactam antibiotics? It is enigmatic why this concept is consistently disregarded. Med. Hypotheses, 62, 367–374.CrossRefPubMedGoogle Scholar
  5. Gronroos, J.O., Laine Veli, J.O. and Nevalainen, T.J. (2002) Bactericidal group II? phospholipase ?2in serum of patients with bactericidal infections. J. Infect. Dis. 185, 1767–1772.CrossRefPubMedGoogle Scholar
  6. Jankowski, S. (1995) The role of complement and antibodies in impaired bactericidal activity of neonatal sera against gram-negative bacteria. Acta Microbiol. Pol. 44, 5–14.PubMedGoogle Scholar
  7. Li, Y.M. (1998) Glycation ligand binding motif in lactoferrin. Implications in diabetic infection. Adv. Exp. Med. Biol. 443, 57–63.PubMedGoogle Scholar
  8. Muschel, L.H. and Treffers, H.P. (1956) Quantitative studies on the bactericidal actions of serum and complement. A rapid photometric growth assay for bactericidal activity. J. Immunol. 76, 11–20.PubMedGoogle Scholar
  9. Nieuw Amerongen, A.V. and Veerman, E.C.I. (2002) Saliva the defender or oral cavity. Oral Dis. 8, 12–22.CrossRefGoogle Scholar
  10. Sorensen, O. and Borregaard, N. (1999) Methods for quantitation of human neutrophil proteins, a survey. J. Immunol. Methods 232,179–190.CrossRefPubMedGoogle Scholar
  11. Tanida, T, Okamoto, T, Okamoto, A, Wang, H, Hamata, T, Ueta, E. and Osaki T. (2003) Decreased excretion of antimicrobial proteins and peptides in saliva of patients with oral candidiasis. Oral Pathol. Med. 32, 586–594.CrossRefGoogle Scholar
  12. Taylor, P.W. (1983) Bactericidal and bacteriolytic activity of serum against Gram-negative bacteria. Microbiol. Rev. 47, 46–83.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.National Research Center Institute of Immunology, FMBAMoscowRussia

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