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Triptolide Alters Mitochondrial Functions

  • Ying Su
  • Shanmin Yang
  • Zhenyu Xiao
  • Wei Wang
  • Paul Okunieff
  • Lurong Zhang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 599)

Abstract

Triptolide (TPL), a small molecule purified from the herb Tripterygium wilfordii, has potential clinical application for suppression of chronic autoimmune disorders and inhibition of tumor growth. However, its mechanism of action is largely unknown. In this study, the effect of TPL on mitochondria was explored with a panel of molecular probes that detect the alteration of mitochondrial functions. When Lewis lung carcinoma (LLC) cells were treated with different doses of TPL for four hours, impaired mitochondrial functions were detected. This included an increased production of reactive oxygen species, the opening of the transition pore of mitochondria, the depolarization of the mitochondria membrane, the inhibition of the production of ATP and increased release of ATP as well as the induction of apoptosis. It is likely that by impairment of mitochondrial function, TPL exerts its inhibitory effect on growth of tumor and progression of inflammatory disease.

Keywords

Pulmonary Arterial Hypertension Mitochondrial Function Annexin Versus Mitochondrial Permeability Transition Pore Lewis Lung Cancer Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Ying Su
    • 1
  • Shanmin Yang
    • 1
  • Zhenyu Xiao
    • 1
  • Wei Wang
    • 1
  • Paul Okunieff
    • 1
  • Lurong Zhang
    • 1
  1. 1.Department of Radiation OncologyUniversity of Rochester Medical CenterRochesterUSA

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