Separation Of Protein C From Cohn Fraction Iv-1 By Mini-Antibody

  • Samin Rezania
  • Doh G. Ahn
  • Kyung A. Kang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 599)


Human protein C (PC) is a natural anticoagulant, antithrombotic, anti-inflammatory, and anti-apoptotic in the bloodstream. PC deficiency can lead to abnormal blood clot formation inside blood vessels, possibly causing heart attack, stroke, skin necrosis, or even death. PC can be, therefore, a valuable therapeutic with little side effect, unlike the currently used anti-coagulants. To reduce the cost involved in immuno purification of PC from blood plasma, single chain variable fragments (mini-Mab) are being produced by recombinantE. coli using phagemid technique. As an economic means of purifying the PC specific mini-Mab, metal affinity chromatography (IMAC) purification process was also investigated. Then using the purified mini-Mab, the feasibility of PC purification from the Cohn Fraction IV-1 was examined. Cohn Fraction IV-1 is usually a discarded side-stream from the blood plasma fractionation of human serum albumin. It holds 90% of PC in plasma, but is very cheap. Preliminary study of PC purification from the Cohn Fraction IV-1 showed 16% purification yield using mini-Mab immobilized NHS-activated Sepharose. The economic analysis for PC purification using mini-Mab showed that the overall process was found to be tens of times cheaper than that using Mab.


Human Serum Albumin Elution Step Single Chain Variable Fragment Purification Yield Urea Hydrogen Peroxide 
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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Samin Rezania
    • 1
  • Doh G. Ahn
    • 1
  • Kyung A. Kang
    • 1
  1. 1.Department of Chemical EngineeringUniversity of LouisvilleLouisvilleUSA

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