Pseudogenes and The Electron Transport Chain
With the advent of easy access to the human genome sequence, molecular biology techniques to target respirome-specific genes have begun to be exploited in the study of human disorders and in particular human cancers. In some recent publications it would appear that some investigators have inappropriately targeted pseudogenes rather than functional genes. The high transcription level and generally small size of many of the genes in the respirome make them prone to duplications in the form of processed pseudogenes within the human genome. Such genes can be challenging to analyse using standard molecular genetics approaches. In this presentation, we offer an analysis of pseudogenes that have been identified to have significant homology with some elements of the respirome. Other sequence elements such as Alu repeats, which present similar research obstacles, are also discussed.
KeywordsElectron Transport Chain Ubiquinone Oxidoreductase Insertion Rate Mitochondrial Proteome High Transcription Level
Unable to display preview. Download preview PDF.
- 13.International Human Genome Sequencing Consortium. Initial sequencing and analysis of the human genome. Nature 409, 860-921 (2001).Google Scholar
- 31.U. Weidner, S. Geier, A. Ptock, T. Friedrich, H. Leif and H. Weiss. The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I. J Mol Biol. 233(1), 109-122 (1993).PubMedCrossRefGoogle Scholar
- 34.A. Khelifi, L. Duret and D. Mouchiroud. HOPPSIGEN: a database of human and mouse processed pseudogenes. Nucleic Acids Res. 33(Database issue), D59-D66 (2005).Google Scholar
- 35.K.A. Kreuzer, U. Lass, O. Landt, A. Nitsche, J. Laser, H. Ellerbrok, G. Pauli, D. Huhn and C.A Schmidt. Highly sensitive and specific fluorescence reverse transcription-PCR assay for the pseudogene-free detection of beta-actin transcripts as quantitative reference. Clin Chem.45(2), 297-300 (1999).PubMedGoogle Scholar